ROP1 Antibody (YA4619)

Cat. No.: HY-P84922: Data Sheet COA
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ROP1 Antibody (YA4619) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ROP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ROP1 Antibody (YA4619) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ROP1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 42.6 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

A9JQX2

Gene ID

Immunogen

Purified recombinant fragment of Toxoplasma gondii rhoptry protein ROP1 (AA: 42-183) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAW (lane 2(20μg), MCF-7 (lane 3(20μg), L929 (lane 4(20μg) and NIH/3T3 (lane 5(20μg) using ROP1 Antibody (HY-P84922) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (PubMed:14966267, PubMed:15356101, PubMed:15927447, PubMed:21343298, PubMed:25062272). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (PubMed:10869423, PubMed:11032835, PubMed:15155719, PubMed:15572675, PubMed:15927447, PubMed:16019190, PubMed:16179589, PubMed:16230378, PubMed:16324151, PubMed:25062272). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (PubMed:16019190). May regulate insulin signaling cascade (PubMed:14966267). May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB (PubMed:15356101, PubMed:15572675). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (PubMed:16324151). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (PubMed:39909992); NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:15721269, PubMed:23393389). Involved in redox signaling in vascular cells (PubMed:23393389). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage (PubMed:23393389); Lacks superoxide-generating NADPH oxidase activity

Subcellular Localization：Cytoplasm; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cell junction, focal adhesion; Nucleus; Nucleus; Nucleus, nucleolus; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region

Expression：
Tissue_specificity:This gene is expressed (protein level) in distal renal tubular cells and endothelial cells of the renal cortex. Expression is widespread. It is highly expressed in the kidneys, and expressed at lower levels in the heart, adipocytes, liver cancer cells, endothelial cells, skeletal muscle, brain, various brain tumor cell lines, and airway epithelial cells.

Induction:By 7-ketocholesterol (at protein level)

Isoforms & Post-Translational Modification：Q9NPH5 has 9 isomers: Q9NPH5-1: 66932 Da (predicted); Q9NPH5-2: 58410 Da (predicted); Q9NPH5-3: 27625 Da (predicted); Q9NPH5-4: 31812 Da (predicted); Q9NPH5-5: 25761 Da (predicted); Q9NPH5-6: 62745 Da (predicted); Q9NPH5-7: 6516 Da (predicted); Q9NPH5-8: 64102 Da (predicted); Q9NPH5-9: 59915 Da (predicted).
Deubiquitinated by USP19;N-glycosylated and glycosylation is required for its proper function;N-glycosylated

Subunit：Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with PPP1R15A (PubMed:26742780). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (PubMed:39909992)

RRID

AB_3719002

Synonyms

Toxoplasma gondii rhoptry protein ROP1

Documentation

Select Batch:

Data Sheet (233 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)