Cytochrome P450 Reductase Antibody (YA2619)

Cat. No.: HY-P82874: Data Sheet COA
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User Guide for Antibodies
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Cytochrome P450 Reductase Antibody (YA2619) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 Reductase.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Cytochrome P450 Reductase Antibody (YA2619) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Cytochrome P450 Reductase Antibody (YA2619) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 Reductase.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 77 kDa;

Observed band size: 77 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16435

Gene ID

5447 [NCBI]

Immunogen

A synthetic peptide of human Cytochrome P450 Reductase aa141-154.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from C6(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Cytochrome P450 Reductase Antibody (HY-P82874) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling Cytochrome P450 Reductase Antibody (HY-P82874) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytochrome P450 Reductase Antibody (HY-P82874) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling Cytochrome P450 Reductase Antibody (HY-P82874) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytochrome P450 Reductase Antibody (HY-P82874) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side

RRID

AB_3104967

Database

Entrez Gene: 5447 Human ; 18984 Mouse ; 29441 Rat

SwissProt: P16435 Human ; P37040 Mouse ; P00388 Rat

OMIM: 201750 Human

Research Field

Cardiovascular

Synonyms

CPR; CYPOR; P450R; por

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cytochrome P450 Reductase Antibody (YA2619) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytochrome P450 Reductase Antibody (YA2619)

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Cytochrome P450 Reductase Antibody (YA2619) Related Classifications

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