GRK5 Antibody
(Synonyms: GPRK5, GRK5, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5)GRK5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GRK5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
GRK5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GRK5.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
Synthetic peptide corresponding to the center region of human GRK5.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GRK5 is a Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G protein-coupled receptor, LRP6 during Wnt signaling (in vitro)
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Subcellular Localization
Cytoplasm; Nucleus; Cell membrane
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Expression
Tissue_Specificity: Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Induction: Overexpressed during heart failure. -
Isoforms & Post-Translational Modification
GRK5 has an amino acid length of 590, molecular weight is 67787 Da.发生自磷酸化。自磷酸化可能在 GRK5 激酶活性的调控中发挥关键作用GRK5 的氨基酸个数为 590 个，分子量为 67787 Da。Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity
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Subunit
Interacts with ST13 (via the C-terminus 303-319 AA).
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SwissProt ID
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Synonyms
GPRK5, GRK5, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5
Documentation
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