Cytokeratin 2e Antibody (YA2671)

Cat. No.: HY-P82926: Data Sheet COA
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User Guide for Antibodies
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Cytokeratin 2e Antibody (YA2671) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 2e.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Cytokeratin 2e Antibody (YA2671) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

제품 설명

Cytokeratin 2e Antibody (YA2671) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 2e.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 65 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P35908

Gene ID

3849 [NCBI]

Immunogen

A synthesized peptide derived from human Cytokeratin 2e aa260-440/639.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg) using Cytokeratin 2e Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Cytokeratin 2e Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Probably contributes to terminal cornification (PubMed:1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329). Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is expressed in the upper spinous and granular layers of normal adult epidermal tissue in most body parts, including the thighs, nipples, soles of the feet, penile shaft, and armpits. It is not expressed in the foreskin, squamous metaplasia, or carcinoma. In hypertrophic scars and keloids, expression begins in the deepest upper layers. Expression is weak in normal gingiva and tongue, but is enhanced in benign keratosis of the lingual mucosa and mild to moderate oral dysplasia with orthokeratosis.

Induction:Induced in keratinocytes by all-trans retinoic acid (ATRA) , via increase in mRNA stability

Subunit：Heterotetramer of two type I and two type II keratins. Associates with KRT10

RRID

AB_3105018

Database

Entrez Gene: 3849 Human

SwissProt: P35908 Human

OMIM: 146800 Human

Research Field

Cell Biology

Synonyms

KRT2; KRT76; KRT3; KRT5; KRT6A; KRT6B; KRT6C; KRT71; KRT72; KRT73; KRT74; KRT75; KRT79; KRT8; KRT84; Keratin; type II cytoskeletal 2 epidermal; Keratin; type II cytoskeletal 2 oral; Keratin; type II cytoskeletal 3; Keratin; type II cytoskeletal 5; Keratin; type II cytoskeletal 6A; Keratin; type II cytoskeletal 6B; Keratin; type II cytoskeletal 6C; Keratin; type II cytoskeletal 71; Keratin; type II cytoskeletal 72; Keratin; type II cytoskeletal 73; Keratin; type II cytoskeletal 74

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/kg-1-cell.html [Content Brief]

Cytokeratin 2e Antibody (YA2671) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytokeratin 2e Antibody (YA2671)

Cytokeratin 2e Antibody (YA2671) Featured Recommendations:

Product Detail

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Background

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References

Cytokeratin 2e Antibody (YA2671) Related Classifications

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