Copy Link Print Information

PRMT5 Antibody (YA1853)

(Synonyms: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5; Skb1Hs Methyltransferase)
Customer Review

Based on 1 Customer Validation

PRMT5 Antibody (YA1853) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P FC
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • PRMT5 Antibody (YA1853) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

PRMT5 Antibody (YA1853) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 73 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PRMT5 aa40-92.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3675821

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293(lane 3(20ug) and Raji(lane 4(20ug)using PRMT5 Antibody (HY-P82108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling PRMT5 Antibody (YA1853) (HY-P82108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Protein arginine methyltransferase 5 (PRMT5) is a type II arginine methyltransferase that catalyzes symmetric dimethylation of arginine residues on histone and non-histone substrates, thereby regulating chromatin organization, transcriptional control, and cellular development[1][2]. PRMT5 functions as a major epigenetic regulator through symmetric methylation marks such as H3R8me2s and H4R3me2s, which influence gene expression programs and growth-regulatory pathways[1][3]. Mechanistically, PRMT5 plays a central role in RNA processing and alternative splicing, and has been identified as a master regulator of mammalian splicing networks, linking arginine methylation to transcriptome regulation[4][5]. Therefore, PRMT5 integrates transcriptional and post-transcriptional regulation to control cell proliferation, differentiation, survival, and genome maintenance[1][5]. In disease settings, PRMT5 is frequently overexpressed or hyperactivated in multiple cancers, where it supports tumor cell growth, metastasis, and therapeutic resistance through regulation of oncogenic signaling, transcription, and RNA metabolism[2][5][6]. Compared with related isoforms, PRMT5 belongs to the type II PRMT family and primarily generates symmetric dimethylarginine, whereas PRMT1 is the major type I enzyme responsible for asymmetric dimethylarginine formation, highlighting distinct but partially overlapping biological functions[3][7]. For experimental applications, PRMT5 has emerged as a therapeutic target, and pharmacological inhibition disrupts methyltransferase-dependent signaling and splicing pathways; recent MTA-cooperative inhibitors further exploit vulnerabilities in MTAP-deleted tumors[5][6]. These characteristics make PRMT5 an important model for studying epigenetic regulation, RNA splicing, and targeted cancer therapy[1][4][6].

  • Subcellular Localization

    Cytoplasm; Nucleus; Chromosome; Golgi apparatus

  • Expression


    Tissue_specificity:Ubiquitous

  • Isoforms & Post-Translational Modification

    O14744 has 5 isomers: O14744-1: 72684 Da (predicted); O14744-2: 71320 Da (predicted); O14744-4: 53580 Da (predicted); O14744-5: 67674 Da (predicted); O14744-3: 66311 Da (predicted).

  • Subunit

    Forms, at least, homodimers and homotetramers (PubMed:11152681). Component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A (PubMed:21081503, PubMed:23071334, PubMed:33376131).

  • SwissProt ID

    O14744

  • Gene ID
    10419 [NCBI]
  • Synonyms

    HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5; Skb1Hs Methyltransferase

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Stopa N, et al. The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond. Cell Mol Life Sci. 2015 Jun;72(11):2041-59. [Content Brief]

[2]. Lee J, et al. Targeting PRMT5 in cancer: Mechanistic insights and clinical progress. Biomed Pharmacother. 2025 Dec;193:118754. [Content Brief]

[3]. Shailesh H, et al. Protein arginine methyltransferase 5 (PRMT5) dysregulation in cancer. Oncotarget. 2018 Nov 30;9(94):36705-36718. [Content Brief]

[4]. Bezzi M, et al. Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery. Genes Dev. 2013 Sep 1;27(17):1903-16. [Content Brief]

[5]. Kim H, et al. PRMT5 function and targeting in cancer. Cell Stress. 2020 Jul;4(8):199-215. [Content Brief]

[6]. Hwang JW, et al. Protein arginine methyltransferases: promising targets for cancer therapy. Exp Mol Med. 2021 May;53(5):788-808. [Content Brief]

[7]. Gao G, et al. PRMT1 loss sensitizes cells to PRMT5 inhibition. Nucleic Acids Res. 2019 Jun 4;47(10):5038-5048. [Content Brief]

View More

PRMT5 Antibody (YA1853) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293(lane 3(20ug) and Raji(lane 4(20ug)using PRMT5 Antibody (HY-P82108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling PRMT5 Antibody (YA1853) (HY-P82108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PRMT5 Antibody (YA1853) Product Image
    1/16
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • PRMT5 Antibody (YA1853) Product Image
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293(lane 3(20ug) and Raji(lane 4(20ug)using PRMT5 Antibody (HY-P82108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling PRMT5 Antibody (YA1853) (HY-P82108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • Experimental Validation Results for PRMT5 Antibody (YA1853)
  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293(lane 3(20ug) and Raji(lane 4(20ug)using PRMT5 Antibody (HY-P82108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with PRMT5 Antibody (HY-P82108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRMT5 Antibody (HY-P82108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PRMT5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT5 Antibody (YA1853) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling PRMT5 Antibody (YA1853) (HY-P82108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PRMT5 Antibody (YA1853) Product Image WB
  • PRMT5 Antibody (YA1853) Product Image ICC
  • PRMT5 Antibody (YA1853) Product Image ICC
  • PRMT5 Antibody (YA1853) Product Image IHC-P
  • PRMT5 Antibody (YA1853) Product Image IHC-P
  • PRMT5 Antibody (YA1853) Product Image FC