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Cyclin E2 Antibody (YA2594)

(Synonyms: CCNE2; G1/S-specific cyclin-E2)
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Based on 1 Customer Validation

Cyclin E2 Antibody (YA2594) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cyclin E2 Antibody (YA2594)
  • Cyclin E2 Antibody (YA2594) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Cyclin E2 Antibody (YA2594) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database

Entrez Gene: 9134 Human

SwissProt: O96020 Human

Immunogen

A synthetic peptide of human Cyclin E2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104942

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin E2 Antibody (YA2594)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon

    Induction:Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively

  • Isoforms & Post-Translational Modification

    O96020 has 2 isomers: O96020-1: 46757 Da (predicted); O96020-2: 41465 Da (predicted).
    Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway;Lactylated at Lys-348 (PubMed:36896611). Delactylated by SIRT3 (PubMed:36896611)

  • Subunit

    Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex

  • SwissProt ID

    O96020

  • Gene ID
    9134 [NCBI]
  • Synonyms

    CCNE2; G1/S-specific cyclin-E2

  • Research Field

    Cell Biology

Cyclin E2 Antibody (YA2594) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cyclin E2 Antibody (YA2594) Product Image
    1/16
  • Experimental Validation Results for Cyclin E2 Antibody (YA2594)
  • Cyclin E2 Antibody (YA2594) Product Image
  • Experimental Validation Results for Cyclin E2 Antibody (YA2594)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cyclin E2 Antibody (YA2594)
  • Cyclin E2 Antibody (YA2594) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cyclin E2 Antibody (YA2594) Product Image WB