Cyclin E2 Antibody (YA2594)

Cat. No.: HY-P82849: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Cyclin E2 Antibody (YA2594) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cyclin E2 Antibody (YA2594) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Cyclin E2 Antibody (YA2594) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O96020

Gene ID

9134 [NCBI]

Immunogen

A synthetic peptide of human Cyclin E2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Essential for the control of the cell cycle at the late G1 and early S phase

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon

Induction:Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively

Isoforms & Post-Translational Modification：O96020 has 2 isomers: O96020-1: 46757 Da (predicted); O96020-2: 41465 Da (predicted).
Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway;Lactylated at Lys-348 (PubMed:36896611). Delactylated by SIRT3 (PubMed:36896611)

Subunit：Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex

RRID

AB_3104942

Database

Entrez Gene: 9134 Human

SwissProt: O96020 Human

OMIM: 603775 Human

Research Field

Cell Biology

Synonyms

CCNE2; G1/S-specific cyclin-E2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cyclin E2 Antibody (YA2594) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cyclin E2 Antibody (YA2594)

Cyclin E2 Antibody (YA2594) Featured Recommendations:

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Background

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Cyclin E2 Antibody (YA2594) Related Classifications

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