2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Glypican 3 Antibody (YA5632)

Glypican 3 Antibody (YA5632)

製品番号: HY-P85940
User Guide for Antibodies Technical Support

Glypican 3 Antibody (YA5632) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glypican 3.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Glypican 3 Antibody (YA5632) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glypican 3.
Host

Mouse
Clonality

Monoclonal
分子量
Predicted band size: 66 kDa;
Observed band size: 66 kDa,58 kDa,22 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1000
WB
WB: Western Blot
1:500-2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:1000-5000
純度 Protein G Conjugation Non-conjugated
Modification Unmodified Isotype IgG1
Appearance

Liquid
Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Cell surface proteoglycan (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity)
Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
Expression：
Tissue_specificity:Protein levels were detected in the placenta (PubMed: 32337544) . High expression was observed in the lungs, liver, and kidneys.
Isoforms & Post-Translational Modification：P51654 has 3 isomers: P51654-1: 65563 Da (predicted); P51654-2: 59475 Da (predicted); P51654-3: 68414 Da (predicted).
O-glycosylated; contains heparan sulfate and/or chondroitin sulfate;Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor (PubMed:14610063). This processing is essential for its role in inhibition of hedgehog signaling (PubMed:25653284). A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface (PubMed:14610063)
Subunit：Heterodimer; disulfide-linked (PubMed:14610063). Cleavage by a furin-like convertase results in production of alpha and beta chains which form a disulfide-linked heterodimer (PubMed:14610063). Interacts with DPP4 (PubMed:17549790). Interacts with FGF2 (By similarity). Interacts with WNT5A (PubMed:14610063). Also interacts with WNT3A and WNT7B (PubMed:16227623). Interacts with hedgehog protein SHH; the heparan sulfate chains are not required for the interaction (By similarity). Also interacts with hedgehog protein IHH (By similarity). Interacts with CD81 (By similarity). Interacts with Wnt receptors FZD4, FZD7 and FZD8; the heparan sulfate chains are required for the interaction (PubMed:24496449)
Synonyms
DGSX; Glypican proteoglycan 3; Glypican-3 [Precursor]; Gpc3; GPC3_HUMAN; GTR2 2; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI 5; Intestinal protein OCI-5; MXR7; OCI 5; OCI-5; OCI5; SDYS; Secreted glypican-3; SGB; SGBS; SGBS1
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Glypican 3 Antibody (YA5632) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Glypican 3 Antibody (YA5632)
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HY-P85940
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