PRPF31 Antibody (YA3338)

製品番号: HY-P83593: データシート SDS COA User Guide for Antibodies
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PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

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容量	価格（税別）	在庫状況	数量
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8WWY3

Gene ID

26121 [NCBI]

Immunogen

A synthetic peptide of human PRPF31 aa 380-393.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543)

Subcellular Localization：Nucleus; Nucleus speckle; Nucleus, Cajal body

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：Q8WWY3 has 4 isomers: Q8WWY3-1: 55456 Da (predicted); Q8WWY3-2: 40779 Da (predicted); Q8WWY3-3: 30966 Da (predicted); Q8WWY3-4: 54909 Da (predicted).
Phosphorylated by PRP4K during spliceosome assembly

Subunit：Identified in the spliceosome B complex (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:11867543, PubMed:16723661, PubMed:26912367).

Database

Entrez Gene: 26121 Human ; 68988 Mouse ; 292536 Rat

SwissProt: Q8WWY3 Human ; Q8CCF0 Mouse ;

OMIM: 600138 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Protein 61K; hPrp31; U4/U6 snRNP 61 kDa protein

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]