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PRPF31 Antibody (YA3338)

(Synonyms: Protein 61K; hPrp31; U4/U6 snRNP 61 kDa protein)
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Based on 1 Customer Validation

PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PRPF31 Antibody (YA3338)
  • PRPF31 Antibody (YA3338) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PRPF31 aa 380-393.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRPF31 Antibody (YA3338)
    Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PRPF31 is involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome[1][2][3].

  • Subcellular Localization

    Nucleus; Nucleus speckle; Nucleus, Cajal body

  • Expression


    Tissue_specificity:Ubiquitously expressed

  • Isoforms & Post-Translational Modification

    Q8WWY3 has 4 isomers: Q8WWY3-1: 55456 Da (predicted); Q8WWY3-2: 40779 Da (predicted); Q8WWY3-3: 30966 Da (predicted); Q8WWY3-4: 54909 Da (predicted).
    Phosphorylated by PRP4K during spliceosome assembly

  • Subunit

    Identified in the spliceosome B complex (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:11867543, PubMed:16723661, PubMed:26912367).

  • SwissProt ID

    Q8WWY3

  • Gene ID
    26121 [NCBI]
  • Synonyms

    Protein 61K; hPrp31; U4/U6 snRNP 61 kDa protein

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Makarova OV, et al. Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing. EMBO J. 2002 Mar 1;21(5):1148-57. [Content Brief]

[2]. Schneider M, et al. Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation. Nat Struct Mol Biol. 2010 Feb;17(2):216-21. [Content Brief]

[3]. Bertram K, et al. Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. Cell. 2017 Aug 10;170(4):701-713.e11. [Content Brief]

PRPF31 Antibody (YA3338) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PRPF31 Antibody (YA3338) Product Image
    1/16
  • Experimental Validation Results for PRPF31 Antibody (YA3338)
  • PRPF31 Antibody (YA3338) Product Image
  • Experimental Validation Results for PRPF31 Antibody (YA3338)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PRPF31 Antibody (YA3338)
  • PRPF31 Antibody (YA3338) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PRPF31 Antibody (YA3338) Product Image WB