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RSL1D1 Antibody (YA9016)

(Synonyms: CATX11, CSIG, PBK1, L12, RSL1D1, Ribosomal L1 domain-containing protein 1, CATX-11, Cellular senescence-inhibited gene protein, Protein PBK1)
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Based on 1 Customer Validation

RSL1D1 Antibody (YA9016) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RSL1D1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RSL1D1 Antibody (YA9016)
  • RSL1D1 Antibody (YA9016) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:30-3000

Product Details

Description

RSL1D1 Antibody (YA9016) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RSL1D1.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

A synthesized peptide derived from human RSL1D1.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RSL1D1 Antibody (YA9016)
    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), K562 (lane 3), HeLa (lane 4, Negative), Raji (lane 5, Negative), HT-1080 (lane 6), and THP-1 (lane 7) using RSL1D1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    RSL1D1 regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage

  • Subcellular Localization

    Nucleus, nucleolus

  • Expression


    Tissue_Specificity: Expressed at high intensities in the heart, skeletal muscle, and placenta.
    Induction: Down-regulated during cellular senescence.

  • Isoforms & Post-Translational Modification

    RSL1D1 has 2 isoforms, O76021-1: amino acid length is 490, molecular weight is 54973 Da (predicted); O76021-2: amino acid length is 270, molecular weight is 30354 Da (predicted).

  • Subunit

    Interacts with ING1 (isoform 2).

  • SwissProt ID

    O76021

  • Gene ID
    26156 [NCBI]
  • Synonyms

    CATX11, CSIG, PBK1, L12, RSL1D1, Ribosomal L1 domain-containing protein 1, CATX-11, Cellular senescence-inhibited gene protein, Protein PBK1

RSL1D1 Antibody (YA9016) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), K562 (lane 3), HeLa (lane 4, Negative), Raji (lane 5, Negative), HT-1080 (lane 6), and THP-1 (lane 7) using RSL1D1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RSL1D1 Antibody (YA9016) Product Image
    1/16
  • Experimental Validation Results for RSL1D1 Antibody (YA9016)
  • RSL1D1 Antibody (YA9016) Product Image
  • Experimental Validation Results for RSL1D1 Antibody (YA9016)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), K562 (lane 3), HeLa (lane 4, Negative), Raji (lane 5, Negative), HT-1080 (lane 6), and THP-1 (lane 7) using RSL1D1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for RSL1D1 Antibody (YA9016)
  • RSL1D1 Antibody (YA9016) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), K562 (lane 3), HeLa (lane 4, Negative), Raji (lane 5, Negative), HT-1080 (lane 6), and THP-1 (lane 7) using RSL1D1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RSL1D1 Antibody (YA9016) Product Image WB