PAK2 Antibody (YA5004)

Cat. No.: HY-P85312: Ficha de datos User Guide for Antibodies
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PAK2 Antibody (YA5004) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PAK2.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Precio	Stock
20 μL		Pide un presupuesto y un plazo de entrega
50 μL		Pide un presupuesto y un plazo de entrega
100 μL		Pide un presupuesto y un plazo de entrega

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

PAK2 Antibody (YA5004) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PAK2.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 58 kDa;

Observed band size: 61 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13177

Gene ID

5062 [NCBI]

Immunogen

A synthesized peptide derived from human PAK2. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Pureza	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed:12853446, PubMed:16617111, PubMed:19273597, PubMed:19923322, PubMed:33693784, PubMed:7744004, PubMed:9171063). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed:7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed:7744004). Full-length PAK2 stimulates cell survival and cell growth (PubMed:7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed:21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed:21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed:21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed:21555521, PubMed:27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed:19273597, PubMed:19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed:12853446, PubMed:16617111, PubMed:9171063). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed:15234964)

Subcellular Localization：Cytoplasm; Nucleus; Nucleus; Cytoplasm, perinuclear region; Membrane; Lipid-anchor

Expression：
Tissue_specificity:Widely expressed. High expression levels are found in skeletal muscle, ovaries, thymus, and spleen.

Subunit：Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (PubMed:20696164). Interacts with SH3MD4 (PubMed:16374509). Interacts with SCRIB (PubMed:18716323). Interacts with ARHGEF7 and GIT1 (PubMed:19273597). PAK-2p34 interacts with ARHGAP10 (PubMed:15471851)

Synonyms

PAK2; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]