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MAD2L1 Binding Protein Antibody (YA3063)

(Synonyms: MAD2L1BP; CMT2; KIAA0110; MAD2L1-binding protein; Caught by MAD2 protein)
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Based on 1 Customer Validation

MAD2L1 Binding Protein Antibody (YA3063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD2L1 Binding Protein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200

Product Details

Description

MAD2L1 Binding Protein Antibody (YA3063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD2L1 Binding Protein.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MAD2L1BP aa200-250/274.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) , SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) , MCF-7(lane 5(20ug) using MAD2L1 Binding Protein Antibody(HY-P83318) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MAD2L1 Binding Protein may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex

  • Subcellular Localization

    Nucleus; Cytoplasm, cytoskeleton, spindle

  • Isoforms & Post-Translational Modification

    Q15013 has 2 isomers: Q15013-1: 31052 Da (predicted); Q15013-3: 34676 Da (predicted).

  • Subunit

    Interacts with MAD2L1

  • SwissProt ID

    Q15013

  • Gene ID
    9587 [NCBI]
  • Synonyms

    MAD2L1BP; CMT2; KIAA0110; MAD2L1-binding protein; Caught by MAD2 protein

  • Research Field

    Cell Biology

MAD2L1 Binding Protein Antibody (YA3063) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) , SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) , MCF-7(lane 5(20ug) using MAD2L1 Binding Protein Antibody(HY-P83318) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
    1/16
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) , SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) , MCF-7(lane 5(20ug) using MAD2L1 Binding Protein Antibody(HY-P83318) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • Experimental Validation Results for MAD2L1 Binding Protein Antibody (YA3063)
  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) , SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) , MCF-7(lane 5(20ug) using MAD2L1 Binding Protein Antibody(HY-P83318) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAD2L1 Binding Protein Antibody (YA3063) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAD2L1 Binding Protein Antibody (YA3063) Product Image WB
  • MAD2L1 Binding Protein Antibody (YA3063) Product Image ICC
  • MAD2L1 Binding Protein Antibody (YA3063) Product Image ICC