MAD2L1 Binding Protein Antibody (YA3063)

Cat. No.: HY-P83318: Data Sheet COA
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MAD2L1 Binding Protein Antibody (YA3063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD2L1 Binding Protein.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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MAD2L1 Binding Protein Antibody (YA3063) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

MAD2L1 Binding Protein Antibody (YA3063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD2L1 Binding Protein.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 31 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15013

Gene ID

9587 [NCBI]

Immunogen

A synthesized peptide derived from human MAD2L1BP aa200-250/274.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from RAW264.7(lane 2(20ug) , SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) , MCF-7(lane 5(20ug) using MAD2L1 Binding Protein Antibody(HY-P83318) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling MAD2L1 Binding Protein Antibody with MAD2L1 Binding Protein Antibody (HY-P83318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAD2L1 Binding Protein Antibody ((HY-P83318) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex

Subcellular Localization：Nucleus; Cytoplasm, cytoskeleton, spindle

Isoforms & Post-Translational Modification：Q15013 has 2 isomers: Q15013-1: 31052 Da (predicted); Q15013-3: 34676 Da (predicted).

Subunit：Interacts with MAD2L1

Database

Entrez Gene: 9587 Human ; 66591 Mouse ; 316237 Rat

SwissProt: Q15013 Human ; Q9DCX1 Mouse ;

OMIM: 618136 Human

Research Field

Cell Biology

Synonyms

MAD2L1BP; CMT2; KIAA0110; MAD2L1-binding protein; Caught by MAD2 protein

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

MAD2L1 Binding Protein Antibody (YA3063) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MAD2L1 Binding Protein Antibody (YA3063)

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