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Fibronectin Antibody (YA762)

(Synonyms: FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG)
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Based on 1 publication(s) in Google Scholar

Fibronectin Antibody (YA762) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Fibronectin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Fibronectin Antibody (YA762) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Fibronectin Antibody (YA762) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Fibronectin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 285 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 272 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Fibronectin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102658

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fibronectin Antibody (YA762)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Fibronectin Antibody (YA762)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617); Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling; Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix; Secreted

  • Expression


    Tissue_specificity:Fibronectin (protein level) is expressed in the internal limiting membrane of the retina and around blood vessels (PubMed: 29777959) . Plasma fibronectin (soluble dimer form) is secreted by hepatocytes. Cellular fibronectin (dimer or cross-linked polymer form) is produced by fibroblasts, epithelial cells, and other cell types and deposited in the extracellular matrix in fibrous form. Urine contains Ugl-Y1, Ugl-Y2, and Ugl-Y3 (PubMed: 17614963) .

  • Isoforms & Post-Translational Modification

    P02751 has 17 isomers: P02751-15: 272320 Da (predicted); P02751-1: 262625 Da (predicted); P02751-2: 71971 Da (predicted); P02751-3: 259216 Da (predicted); P02751-4: 222976 Da (predicted); P02751-5: 243334 Da (predicted); P02751-6: 240509 Da (predicted); P02751-7: 268912 Da (predicted); P02751-8: 252811 Da (predicted); P02751-9: 246688 Da (predicted); P02751-10: 239626 Da (predicted); P02751-11: 262406 Da (predicted); P02751-12: 221292 Da (predicted); P02751-13: 249322 Da (predicted); P02751-14: 249402 Da (predicted); P02751-16: 73683 Da (predicted); P02751-17: 256502 Da (predicted).
    Sulfated;It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated;Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers);Phosphorylated by FAM20C in the extracellular medium;Proteolytic processing produces the C-terminal NC1 peptide, anastellin;Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation;Serotonylated on Gln residues by TGM2 in response to hypoxia

  • Subunit

    Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 (By similarity). Interacts with COMP (PubMed:12225811). Interacts (via type III repeats 9-14) with TNFAIP6 (via CUB domain); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1 (PubMed:18042364). Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth (By similarity). Interacts with FST3 and MYOC. Interacts with SVEP1 (By similarity)

  • SwissProt ID

    P02751

  • Gene ID
    2335 [NCBI]
  • Synonyms

    FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG

  • Research Field

    Cardiovascular

Fibronectin Antibody (YA762) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Fibronectin Antibody (YA762) Product Image
    1/16
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Fibronectin Antibody (YA762) Product Image
  • Experimental Validation Results for Fibronectin Antibody (YA762)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Fibronectin Antibody (YA762)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Experimental Validation Results for Fibronectin Antibody (YA762)
  • Fibronectin Antibody (YA762) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Fibronectin Antibody (YA762) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Fibronectin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Fibronectin Antibody (YA762) Product Image IHC-P
  • Fibronectin Antibody (YA762) Product Image IHC-P