Stathmin 1 Antibody (YA050)

製品番号: HY-P80349: データシート SDS COA User Guide for Antibodies
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Stathmin 1 Antibody (YA050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Stathmin 1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Stathmin 1 Antibody (YA050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Stathmin 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 17 kDa;

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16949

Gene ID

3925 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Stathmin 1.AA range:100-149.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	純度	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using Stathmin 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Stathmin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Stathmin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:It is widely distributed. The strongest expression is found in the brain, spinal cord, and cerebellum, followed by the thymus, bone marrow, testes, and fetal liver. Moderate expression is found in the colon, ovaries, placenta, uterus, and trachea. It is detectable in all other tissues examined, but at significantly reduced levels. The lowest expression is found in the liver.

Induction:Down-regulated in response to enterovirus 71 (EV71) infection (at protein level)

Subunit：Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST

RRID

AB_3102106

Database

Entrez Gene: 3925 Human ; 16765 Mouse ; 29332 Rat

SwissProt: P16949 Human ; P54227 Mouse ; P13668 Rat

OMIM: 151442 Human

Research Field

Neuroscience

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)