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Phospho-GluR1 (Ser845) Antibody (YA9858)

(Synonyms: GRIA1; GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1)
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Based on 1 Customer Validation

Phospho-GluR1 (Ser845) Antibody (YA9858) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-GluR1 (Ser845).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-GluR1 (Ser845) Antibody (YA9858)
  • Phospho-GluR1 (Ser845) Antibody (YA9858) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:10-50

Product Details

Description

Phospho-GluR1 (Ser845) Antibody (YA9858) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-GluR1 (Ser845).

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 102 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 102 kDa
Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-GluR1 (Ser845) Antibody (YA9858)
    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg) and Rat brain tissue (lane3, 20μg) using Phospho-GluR1 (Ser845) Antibody (HY-P810594). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GluR1 is an Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Resensitization is blocked by CNIH2 tH2O2gh interaction with CACNG8 in the CACNG8-containing AMPA receptors complex. Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+)[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Postsynaptic cell membrane; Multi-pass membrane protein; Postsynaptic density membrane; Multi-pass membrane protein; Cell projection, dendrite; Cell projection, dendritic spine; Early endosome membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein; Presynapse; Synapse

  • Expression


    Tissue_specificity:Widely expressed in the brain

  • Subunit

    Homotetramer or heterotetramer of pore-forming glutamate receptor subunits; heteromeric assembly can be the result of both receptor subtype and flip or flop form and according the composition, one partner can be dominant with respect to the fast desensitizing current component, whereas the other can determine the steady-state component (By similarity). Tetramers may be formed by the dimerization of dimers (PubMed:23739980). Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity). Interacts with HIP1 and RASGRF2. Interacts with SYNDIG1 and GRIA2 (By similarity). Interacts with DLG1 (via C-terminus). Interacts with LRFN1. Interacts with PRKG2. Interacts with CNIH2 and CACNG2 (PubMed:20805473). Interacts with CACNG5; this interaction modulates the gating. Interacts (via C-terminus) with PDLIM4 (via LIM domain); this interaction as well as the interaction of PDLIM4 with alpha-actinin is required for their colocalization in early endosomes (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain) (By similarity). Interacts with CACNG3; associates GRIA1 with the adapter protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons (By similarity). Interacts with CACNG2; this interaction mediates traffick to the plasma membrane and modulation of desensitization. Interacts with CNIH2 and CNIH3; this interaction promotes expression at the plasma membrane and extensively modulates their gating properties by slowing deactivation and desensitization kinetics (By similarity). Found in a complex with GRIA2, GRIA3, GRIA4, DLG4, CACNG8 and CNIH2 (By similarity)

  • SwissProt ID

    P42261

  • Gene ID
    2890 [NCBI]
  • Synonyms

    GRIA1; GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1

Phospho-GluR1 (Ser845) Antibody (YA9858) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg) and Rat brain tissue (lane3, 20μg) using Phospho-GluR1 (Ser845) Antibody (HY-P810594). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-GluR1 (Ser845) Antibody (YA9858) Product Image
    1/16
  • Experimental Validation Results for Phospho-GluR1 (Ser845) Antibody (YA9858)
  • Phospho-GluR1 (Ser845) Antibody (YA9858) Product Image
  • Experimental Validation Results for Phospho-GluR1 (Ser845) Antibody (YA9858)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg) and Rat brain tissue (lane3, 20μg) using Phospho-GluR1 (Ser845) Antibody (HY-P810594). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-GluR1 (Ser845) Antibody (YA9858)
  • Phospho-GluR1 (Ser845) Antibody (YA9858) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg) and Rat brain tissue (lane3, 20μg) using Phospho-GluR1 (Ser845) Antibody (HY-P810594). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-GluR1 (Ser845) Antibody (YA9858) Product Image WB