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SETDB1/ESET Antibody (YA4277)

(Synonyms: ESET; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1)
Customer Review

Based on 1 Customer Validation

SETDB1/ESET Antibody (YA4277) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SETDB1/ESET.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • SETDB1/ESET Antibody (YA4277) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

SETDB1/ESET Antibody (YA4277) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SETDB1/ESET.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 143 kDa
Immunogen

Purified recombinant fragment of human SETDB1 aa 868-1211.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718940

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    Western blot analysis of extracts from A549(lane 1(40μg) )
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    Western blot analysis of extracts from A549(lane 1(40μg) ),K562(lane 2(40μg) ),MCF-7(lane 3(40μg) ),A431(lane 4(40μg) ),Hela(lane 5(40μg) ),HEK293(lane 6(40μg) )and NIH/3T3(lane 7(40μg) ) using SETDB1 antibody(30132P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SETDB1/ESET is a Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions[1][2][3][4][5][6][7].

  • Subcellular Localization

    Nucleus; Cytoplasm; Chromosome

  • Expression


    Tissue_specificity:Widely expressed. Highly expressed in the testes.

  • Isoforms & Post-Translational Modification

    Q15047 has 3 isomers: Q15047-1: 143157 Da (predicted); Q15047-2: 44689 Da (predicted); Q15047-3: 143001 Da (predicted).
    Degraded by the proteasome, shielded by interaction with ATF7IP;Monoubiquitinated at Lys-867 by E2 enzymes of the UBE2E family. The conjugated-Ub is protected from deubiquitination by the SET domain. Monoubiquitination at Lys-867 is required for catalytic activity, H3K9 methylation and endogenous retrovirus silencing

  • Subunit

    Part of a complex containing at least CDYL, REST, WIZ, SETDB1, EHMT1 and EHMT2 (PubMed:19061646). Forms a complex with ATRX, TRIM28 and ZNF274 (PubMed:27029610).

  • SwissProt ID

    Q15047

  • Gene ID
    9869 [NCBI]
  • Synonyms

    ESET; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1

SETDB1/ESET Antibody (YA4277) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 1(40μg) ),K562(lane 2(40μg) ),MCF-7(lane 3(40μg) ),A431(lane 4(40μg) ),Hela(lane 5(40μg) ),HEK293(lane 6(40μg) )and NIH/3T3(lane 7(40μg) ) using SETDB1 antibody(30132P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SETDB1/ESET Antibody (YA4277) Product Image
    1/16
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • SETDB1/ESET Antibody (YA4277) Product Image
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 1(40μg) )
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 1(40μg) ),K562(lane 2(40μg) ),MCF-7(lane 3(40μg) ),A431(lane 4(40μg) ),Hela(lane 5(40μg) ),HEK293(lane 6(40μg) )and NIH/3T3(lane 7(40μg) ) using SETDB1 antibody(30132P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • Experimental Validation Results for SETDB1/ESET Antibody (YA4277)
  • SETDB1/ESET Antibody (YA4277) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 1(40μg) )

  • SETDB1/ESET Antibody (YA4277) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 1(40μg) ),K562(lane 2(40μg) ),MCF-7(lane 3(40μg) ),A431(lane 4(40μg) ),Hela(lane 5(40μg) ),HEK293(lane 6(40μg) )and NIH/3T3(lane 7(40μg) ) using SETDB1 antibody(30132P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SETDB1/ESET Antibody (YA4277) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SETDB1/ESET Antibody (YA4277) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling SETDB1 with SETDB1 Antibody (HY-P84580) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SETDB1 Antibody (HY-P84580) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SETDB1/ESET Antibody (YA4277) Product Image WB
  • SETDB1/ESET Antibody (YA4277) Product Image WB
  • SETDB1/ESET Antibody (YA4277) Product Image ICC
  • SETDB1/ESET Antibody (YA4277) Product Image ICC