Dynamin I Antibody (YA4817)

Cat. No.: HY-P85125: Ficha de datos User Guide for Antibodies
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Dynamin I Antibody (YA4817) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Dynamin I.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

Dynamin I Antibody (YA4817) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Dynamin I.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 97 kDa;

Species Reactivity

Human

SwissProt ID

Q05193

Gene ID

1759 [NCBI]

Immunogen

Purified recombinant fragment of human Dynamin I expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE) (PubMed:15703209, PubMed:20428113, PubMed:29668686, PubMed:8101525, PubMed:8910402, PubMed:9362482). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks (PubMed:30069048, PubMed:7877694, PubMed:9922133). Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis (PubMed:19084269). Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission (PubMed:19084269). May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR (PubMed:29668686). Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses (By similarity). Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity (By similarity). Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells (By similarity). Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP (By similarity). Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation (PubMed:12791276)

Subcellular Localization：Cell membrane; Membrane, clathrin-coated pit; Cytoplasmic vesicle; Presynapse; Cytoplasmic vesicle, secretory vesicle, chromaffin granule

Isoforms & Post-Translational Modification：Q05193 has 4 isomers: Q05193-1: 97408 Da (predicted); Q05193-2: 97263 Da (predicted); Q05193-3: 96041 Da (predicted); Q05193-5: 95895 Da (predicted).
Phosphorylation at Ser-774 by GSK3B/GSK3-beta leads to inactivation of receptor-mediated endocytosis in non-neuronal cells (PubMed:29668686). Dephosphorylation at Ser-774, through the EGFR downstream signaling, leads to activation and regulates early stages of clathrin-mediated endocytosis (CME) (PubMed:29668686). Phosphorylated by CDK5 leading to synaptic vesicle endocytosis (SVE) activation (By similarity)

Subunit：Homodimer; homodimerization is mediated by the dynamin-type G domain which promotes assembly-stimulated GTPase activity (PubMed:20428113, PubMed:26612256).

Synonyms

DNM1; DNM; Dynamin-1

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/kms-12-bm-cell.html [Content Brief]