Cat. No.: HY-P86378

Advanced Verification

MMP-7 Antibody (YA6070)

Name: MMP-7 Antibody (YA6070)
Brand: MedChemExpress
SKU: HY-P86378
Price: 105.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: MMP7; MPSL1; PUMP1; Matrilysin; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase)

Customer Review

Based on 1 Customer Validation

MMP-7 Antibody (YA6070) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP-7.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, IHC-P, ICC/IF, IP, ELISA
Reactivity :
Human

Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Conjugation:
Non-conjugated

ALL WB IHC-P

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Verification Images
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	ELISA ELISA: Enzyme Linked Immunosorbent Assay	IP IP: Immunoprecipitation
Dilution Ratio	1:200-1:1000	1:2000-1:10000	1:200-1:1000	1:5000-1:20000	1:50-1:200

Product Details

Description

MMP-7 Antibody (YA6070) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP-7.

Host Rabbit
Clonality Monoclonal
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 30 kDa

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719142

Product Properties

Appearance

Solution
Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Verification Images

Western blot analysis of extracts from A549 (lane2(20μg), HT29 (lane3(20μg), PC-3 (lane4(20μg) and A431 (lane5(20μg) using MMP-7 Antibody (HY-P86378). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using MMP-7 Antibody (YA6070). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86378, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lung tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skin tissue using MMP-7 Antibody (HY-P86378, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function

MMP-7 (matrix metalloproteinase-7, matrilysin-1) is a secreted zinc-dependent endopeptidase that participates in extracellular matrix remodeling and the proteolytic processing of multiple non-matrix substrates, thereby influencing cell proliferation, apoptosis, epithelial repair, and tissue homeostasis^[1]. Unlike many other matrix metalloproteinases, MMP-7 is one of the smallest family members and lacks the conserved C-terminal hemopexin domain, a structural distinction that contributes to its unique substrate profile and biological functions^[1]. Mechanistically, Wnt/β-catenin signaling is a major upstream regulator of MMP-7 transcription, and MMP-7 is a well-established downstream target of β-catenin/TCF-mediated gene activation^[2]^[3]. Through cleavage of substrates including E-cadherin, Fas ligand, and nephrin, MMP-7 regulates epithelial integrity, cell survival, epithelial-mesenchymal transition, and tissue remodeling processes^[1]. In disease settings, elevated MMP-7 expression is associated with tumor progression, invasion, metastasis, and angiogenesis across multiple cancer types, while persistent induction of MMP-7 is also linked to kidney injury, fibrosis, and chronic kidney disease progression^[1]^[4]. Compared with related isoforms such as MMP-2 and MMP-9, MMP-7 exhibits distinct biological activities; notably, MMP-7 can cleave nephrin and promote proteinuria, a function not reported for MMP-2 or MMP-9^[1]. For experimental applications, selective MMP-7 inhibitors have recently demonstrated efficacy in reducing kidney fibrosis in preclinical models, supporting the use of MMP-7 as both a mechanistic target and a translational biomarker in disease research^[4].
Subcellular Localization

Secreted, extracellular space, extracellular matrix
SwissProt ID

P09237
Gene ID

4316 [NCBI]
Synonyms

MMP7; MPSL1; PUMP1; Matrilysin; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase