TBC1D4 Antibody (YA5924)
(Synonyms: TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160)
Based on 1 Customer Validation
TBC1D4 Antibody (YA5924) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBC1D4.
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Host:
Rabbit
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Isotype:
IgG/Kappa
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:1000-1:4000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
TBC1D4 Antibody (YA5924) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBC1D4.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 160 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 147 kDa
Protein A
Non-conjugated
Unmodified
IgG/Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HeLa (lane 1(40μg)) 、HEK293 (lane 2(40μg)) 、SH-SY5Y (lane 3(40μg)) and Mouse colon (lane 4(40μg)) using TBC1D4 Antibody (HY-P86232) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
TBC1D4 may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake
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Subcellular Localization
Cytoplasm
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Expression
Tissue_specificity:Widely expressed. Isomer 2 is most highly overexpressed in most tissues. Isomer 1 is highly expressed in skeletal muscle and heart, but not detected in liver and adipose tissue. Isomer 2 is strongly expressed in adrenal glands and thyroid gland, and also expressed in lung, kidney, colon, brain, and adipose tissue. Isomer 2 is moderately expressed in skeletal muscle. Expressed in pancreatic islets (including β cells) (protein level) . Expression in the islets of patients with type 2 diabetes is reduced by half compared to the control group. Upregulated expression in T cells of atopic dermatitis. -
Isoforms & Post-Translational Modification
O60343 has 5 isomers: O60343-1: 146563 Da (predicted); O60343-2: 139556 Da (predicted); O60343-3: 145689 Da (predicted); O60343-4: 53850 Da (predicted); O60343-5: 60095 Da (predicted).
Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients -
SwissProt ID
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Synonyms
TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160
Documentation
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