TBC1D4 Antibody (YA5924)

Cat. No.: HY-P86232: Data Sheet COA
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User Guide for Antibodies
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TBC1D4 Antibody (YA5924) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBC1D4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TBC1D4 Antibody (YA5924) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBC1D4.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 147 kDa;

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

O60343

Gene ID

9882 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:4000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Widely expressed. Isomer 2 is most highly overexpressed in most tissues. Isomer 1 is highly expressed in skeletal muscle and heart, but not detected in liver and adipose tissue. Isomer 2 is strongly expressed in adrenal glands and thyroid gland, and also expressed in lung, kidney, colon, brain, and adipose tissue. Isomer 2 is moderately expressed in skeletal muscle. Expressed in pancreatic islets (including β cells) (protein level) . Expression in the islets of patients with type 2 diabetes is reduced by half compared to the control group. Upregulated expression in T cells of atopic dermatitis.

Isoforms & Post-Translational Modification：O60343 has 5 isomers: O60343-1: 146563 Da (predicted); O60343-2: 139556 Da (predicted); O60343-3: 145689 Da (predicted); O60343-4: 53850 Da (predicted); O60343-5: 60095 Da (predicted).
Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients

Synonyms

TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)