eIF4EBP2 Antibody (YA1079)(PBS only)
(Synonyms: 4EBP2; PHASII)eIF4EBP2 Antibody (YA1079) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP2.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
eIF4EBP2 Antibody (YA1079) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP2.
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Host Mouse
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 15-20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 13 kDa
Recombinant protein of human 4E-BP2.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
eIF4EBP2 is a Repressor of translation initiation involved in synaptic plasticity, learning and memory formation. Regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form of EIF4EBP2 competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. EIF4EBP2 is enriched in brain and acts as a regulator of synapse activity and neuronal stem cell renewal via its ability to repress translation initiation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways[1][2].
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Subcellular Localization
Cytoplasm; Nucleus
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Subunit
Hypophosphorylated EIF4EBP2 interacts with EIF4E; phosphorylation of EIF4EBP2 by mTORC1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:21661078, PubMed:24207126, PubMed:25533957, PubMed:7935836).
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SwissProt ID
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Synonyms
4EBP2; PHASII
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Tsang B, et al. Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation. Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4218-4227. [Content Brief]
[2]. Bah A, et al. Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch. Nature. 2015 Mar 5;519(7541):106-9. [Content Brief]
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