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USP28 Antibody (YA1336)

(Synonyms: KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin thioesterase 28; UBP28)
Customer Review

Based on 1 Customer Validation

USP28 Antibody (YA1336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP28.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC FC
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • USP28 Antibody (YA1336) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

USP28 Antibody (YA1336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP28.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 135 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 122 kDa
Species Reactivity Database

Entrez Gene: 57646 Human

SwissProt: Q96RU2 Human

Immunogen

A synthesized peptide derived from human USP28 aa110-165/1077.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103703

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for USP28 Antibody (YA1336)
    Western blot analysis of extracts from A431 (lane 2(20μg) , HEK293T (lane 3(20μg) and Hela (lane 4(20μg) using USP28 (HY-P81591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for USP28 Antibody (YA1336)
    Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for USP28 Antibody (YA1336)
    Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for USP28 Antibody (YA1336)
    Flow cytometric analysis of 1X10^6 Hela cells labeling USP28 Antibody (YA1336) (HY-P81591, red). Cells were fixed with 4% paraformaldehyde and permeabilised with intranuclear rupture fluid. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    USP28 is a crucial deubiquitinase (DUB) in the ubiquitin-proteasome system and is highly expressed in many types of human cancer. USP28 is the third DUB, only after OTUB1 and USP7, which has been successfully hijacked to generate effective DUBTACs. Moreover, USP28 has been shown to regulate the stability of several critical proteins involved in the DNA damage response, such as 53BP1 and Claspin, thereby influencing the ability of the cell to repair DNA damage and maintain genomic integrity. The aberrant high expression or activity of USP28 is linked to various types of cancer, including breast, colorectal, and lung cancers, making USP28 a potential cancer drug target.

  • Subcellular Localization

    Nucleus, nucleoplasm

  • Expression


    Induction:Down-regulated upon hypoxia (PubMed:20046830) . Up-regulated by the transcription factor c-Jun/JUN in a KRAS-dependent manner in colorectal cancer (CRC) cells (PubMed:24623306)

  • Isoforms & Post-Translational Modification

    Q96RU2 has 3 isomers: Q96RU2-1: 122491 Da (predicted); Q96RU2-2: 119121 Da (predicted); Q96RU2-3: 66243 Da (predicted).
    Degraded upon nickel ion level or hypoxia exposure;Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR (PubMed:16901786). Phosphorylated by PRKD1 (PubMed:24623306)

  • Subunit

    Interacts with ZNF304 (PubMed:24623306). Interacts with PRKD1 (PubMed:24623306). Interacts with TP53BP1 (PubMed:16901786). Interacts with isoform 1 of FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC (PubMed:17558397, PubMed:17873522)

  • SwissProt ID

    Q96RU2

  • Gene ID
    57646 [NCBI]
  • Synonyms

    KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin thioesterase 28; UBP28

  • Research Field

    Cell Biology

References

[1]. Serra RW, et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife. 2014 Mar 12;3:e02313. [Content Brief]

[2]. Wang Z, et al. USP28-Based Deubiquitinase-Targeting Chimeras for Cancer Treatment. J Am Chem Soc. 2025 Apr 23;147(16):13754-13763. [Content Brief]

USP28 Antibody (YA1336) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg) , HEK293T (lane 3(20μg) and Hela (lane 4(20μg) using USP28 (HY-P81591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling USP28 Antibody (YA1336) (HY-P81591, red). Cells were fixed with 4% paraformaldehyde and permeabilised with intranuclear rupture fluid. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • USP28 Antibody (YA1336) Product Image
    1/16
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • USP28 Antibody (YA1336) Product Image
  • Experimental Validation Results for USP28 Antibody (YA1336)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg) , HEK293T (lane 3(20μg) and Hela (lane 4(20μg) using USP28 (HY-P81591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for USP28 Antibody (YA1336)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for USP28 Antibody (YA1336)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for USP28 Antibody (YA1336)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling USP28 Antibody (YA1336) (HY-P81591, red). Cells were fixed with 4% paraformaldehyde and permeabilised with intranuclear rupture fluid. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • Experimental Validation Results for USP28 Antibody (YA1336)
  • USP28 Antibody (YA1336) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg) , HEK293T (lane 3(20μg) and Hela (lane 4(20μg) using USP28 (HY-P81591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • USP28 Antibody (YA1336) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • USP28 Antibody (YA1336) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling USP28 with USP28 Antibody (HY-P81591) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with USP28 Antibody (HY-P81591) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • USP28 Antibody (YA1336) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling USP28 Antibody (YA1336) (HY-P81591, red). Cells were fixed with 4% paraformaldehyde and permeabilised with intranuclear rupture fluid. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • USP28 Antibody (YA1336) Product Image WB
  • USP28 Antibody (YA1336) Product Image ICC
  • USP28 Antibody (YA1336) Product Image ICC
  • USP28 Antibody (YA1336) Product Image FC