ACAN Antibody (YA6985)

Art. -Nr.: HY-P87302: Data Sheet User Guide for Antibodies
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ACAN Antibody (YA6985) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACAN.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

ACAN Antibody (YA6985) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACAN.

Host

Rabbit

Clonality

Recombinant

Molekulargewicht

Predicted band size: 261 kDa;

Observed band size: 250 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16112

Immunogen

A synthesized peptide derived from human Aggrecan

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:200-2000

Sensitivity	Endogenous	Reinheit	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Expression：
Tissue_Specificity: Detected in fibroblasts (at protein level) (PubMed:36213313). Restricted to cartilage (PubMed:7524681)

Isoforms & Post-Translational Modification：P16112 has four isomers: P16112-1: 261329 Da (predicted); P16112-2: 257441 Da (predicted); P16112-3: 250372 Da (predicted); P16112-4: 265340 Da (predicted).
Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides

Subunit：Forms a complex (via covalent bonds) with MATN1; the interaction increases with age of the organism via an increase in occupancy of MATN1 binding sites (By similarity)

Synonyms

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

Dokumentation

User Guide for Antibodies (1077 KB)