FoxA2 Antibody (YA4863)

Cat. No.: HY-P85171: Data Sheet User Guide for Antibodies
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FoxA2 Antibody (YA4863) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FoxA2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

FoxA2 Antibody (YA4863) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FoxA2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 48 kDa;

Species Reactivity

Human

SwissProt ID

Q9Y261

Gene ID

3170 [NCBI]

Immunogen

Purified recombinant fragment of FoxA2 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HT-29 (lane2(20μg), A549 (lane3(20μg), HepG2 (lane4(20μg) and PC-3 (lane5(20μg) using FoxA2 Antibody (HY-P84884). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation

Subcellular Localization：Nucleus; Cytoplasm

Isoforms & Post-Translational Modification：Q9Y261 has 2 isomers: Q9Y261-1: 48306 Da (predicted); Q9Y261-2: 48907 Da (predicted).
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation

Subunit：Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3 (PubMed:19919681). Interacts with PRKDC (PubMed:19478084). Interacts with AKT1 (By similarity). Interacts with TET1; this interaction may recruit TET1 to specific genomic loci to mediate their demethylation (PubMed:35798741)

Synonyms

FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B

Documentation

User Guide for Antibodies (1077 KB)