FoxA2 Antibody (YA4863)
(Synonyms: FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B)
Based on 1 Customer Validation
FoxA2 Antibody (YA4863) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FoxA2.
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Host:
Mouse
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:10000 | 1:50-200 |
Product Details
FoxA2 Antibody (YA4863) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FoxA2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 48 kDa;
Purified recombinant fragment of FoxA2 expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HT-29 (lane2(20μg), A549 (lane3(20μg), HepG2 (lane4(20μg) and PC-3 (lane5(20μg) using FoxA2 Antibody (HY-P84884). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
FOXA2 belongs to FOXA protein family. During pancreatic genealogy specification, FOXA2 is required to establish the initiation enhancer state and the correct remodeling of chromatin structure to recruit additional transcription factors, such as GATA6, for the activation of the transcriptional program required for human pancreatic differentiation. FOXA2 particularly affects the transition from the hind foregut (FG) to the primary pancreatic progenitor (PP1) stage. Mice with specific deletion of endodermal FOXA2 also exhibited normal growth of the liver. Consistent with the reduction in glucagon gene expression levels, the content of glucagon was reduced in FOXA1-, FOXA2-, and FOXA1/FOXA2-deficient α cells, and it seems that only the combined silencing of FOXA1 and FOXA2 can affect the secretion of glucagon[1].
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Subcellular Localization
Nucleus; Cytoplasm
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Isoforms & Post-Translational Modification
Q9Y261 has 2 isomers: Q9Y261-1: 48306 Da (predicted); Q9Y261-2: 48907 Da (predicted).
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation -
Subunit
Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3 (PubMed:19919681). Interacts with PRKDC (PubMed:19478084). Interacts with AKT1 (By similarity). Interacts with TET1; this interaction may recruit TET1 to specific genomic loci to mediate their demethylation (PubMed:35798741)
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SwissProt ID
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Synonyms
FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B
Documentation
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