DNAJB6 Antibody (YA2704)(PBS only)

Cat. No.: HY-P82959A: Data Sheet User Guide for Antibodies
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DNAJB6 Antibody (YA2704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJB6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DNAJB6 Antibody (YA2704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJB6.

Host

Rabbit

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75190

Gene ID

10049 [NCBI]

Immunogen

Recombinant protein of human DNAJB6 aa162-175.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70 (PubMed:10954706, PubMed:28233300). Plays an indispensable role in the organization of KRT8/KRT18 filaments (PubMed:10954706). Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin (PubMed:11896048, PubMed:22366786). Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (PubMed:20159555, PubMed:22366786). Also reduces cellular toxicity and caspase-3 activity (PubMed:11896048); Isoform B but not isoform A inhibits huntingtin aggregation

Subcellular Localization：Cytoplasm, perinuclear region; Nucleus; Cytoplasm, myofibril, sarcomere, Z line

Expression：
Tissue_specificity:Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus

Isoforms & Post-Translational Modification：O75190 has 4 isomers: O75190-1: 36087 Da (predicted); O75190-2: 26900 Da (predicted); O75190-3: 35714 Da (predicted); O75190-4: 30369 Da (predicted).

Subunit：Homooligomer (PubMed:22366786). Interacts with BAG3, HSPB8 and STUB1 (PubMed:22366786). Interacts with ALKBH1 (By similarity). Interacts with HSP70, KRT18 and PTTG (PubMed:10954706, PubMed:9915854)

Research Field

Neuroscience

Synonyms

DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E

Documentation

User Guide for Antibodies (1077 KB)