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Collagen XVII alpha 1 Antibody (YA2759)

(Synonyms: BP180; BPA 2; BPAG2; COL17A1; Collagen 17; CollagenXVII; LAD1)
Customer Review

Based on 1 Customer Validation

Collagen XVII alpha 1 Antibody (YA2759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen XVII alpha 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Collagen XVII alpha 1 Antibody (YA2759) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:30

Product Details

Description

Collagen XVII alpha 1 Antibody (YA2759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen XVII alpha 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130,180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 150 kDa
Species Reactivity Database

Entrez Gene: 1308 Human ;

SwissProt: Q9UMD9 Human ;

Immunogen

A synthesized peptide derived from human Collagen XVII aa1333-1370.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105132

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
    Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
    Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Collagen XVII alpha 1 may play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane; The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies

  • Subcellular Localization

    Cell junction, hemidesmosome; Membrane; Single-pass type II membrane protein; Secreted, extracellular space, extracellular matrix, basement membrane; Secreted, extracellular space, extracellular matrix, basement membrane

  • Expression


    Tissue_specificity:Detected in skin (PubMed:8618013) . In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728) . Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves

  • Isoforms & Post-Translational Modification

    Q9UMD9 has 2 isomers: Q9UMD9-1: 150419 Da (predicted); Q9UMD9-2: 142344 Da (predicted).
    The intracellular/endo domain is disulfide-linked;Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains;The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544

  • Subunit

    Homotrimers of alpha 1(XVII)chains. Interacts (via cytoplasmic region) with ITGB4 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Interacts (via N-terminus) with PLEC. Interacts (via cytoplasmic region) with DSP

  • SwissProt ID

    Q9UMD9

  • Gene ID
    1308 [NCBI]
  • Synonyms

    BP180; BPA 2; BPAG2; COL17A1; Collagen 17; CollagenXVII; LAD1

  • Research Field

    Signal Transduction

Collagen XVII alpha 1 Antibody (YA2759) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen XVII alpha 1 Antibody (YA2759) Product Image
    1/16
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Collagen XVII alpha 1 Antibody (YA2759) Product Image
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Experimental Validation Results for Collagen XVII alpha 1 Antibody (YA2759)
  • Collagen XVII alpha 1 Antibody (YA2759) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen XVII alpha 1 Antibody (YA2759) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen XVII alpha 1 Antibody (YA2759) Product Image ICC
  • Collagen XVII alpha 1 Antibody (YA2759) Product Image ICC