Collagen XVII alpha 1 Antibody (YA2759)

Cat. No.: HY-P83014: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Collagen XVII alpha 1 Antibody (YA2759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen XVII alpha 1.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
무료 샘플	Apply now
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Collagen XVII alpha 1 Antibody (YA2759) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

Collagen XVII alpha 1 Antibody (YA2759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen XVII alpha 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 150 kDa;

Observed band size: 130,180 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9UMD9

Gene ID

1308 [NCBI]

Immunogen

A synthesized peptide derived from human Collagen XVII aa1333-1370.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:30

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A431 cells labeling Collagen XVII alpha 1 with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Collagen XVII alpha 1 Antibody (HY-P83014) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane; The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies

Subcellular Localization：Cell junction, hemidesmosome; Membrane; Single-pass type II membrane protein; Secreted, extracellular space, extracellular matrix, basement membrane; Secreted, extracellular space, extracellular matrix, basement membrane

Expression：
Tissue_specificity:Detected in skin (PubMed:8618013) . In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728) . Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves

Isoforms & Post-Translational Modification：Q9UMD9 has 2 isomers: Q9UMD9-1: 150419 Da (predicted); Q9UMD9-2: 142344 Da (predicted).
The intracellular/endo domain is disulfide-linked;Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains;The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544

Subunit：Homotrimers of alpha 1(XVII)chains. Interacts (via cytoplasmic region) with ITGB4 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Interacts (via N-terminus) with PLEC. Interacts (via cytoplasmic region) with DSP

RRID

AB_3105132

Database

Entrez Gene: 1308 Human ;

SwissProt: Q9UMD9 Human ;

OMIM: 619787 Human

Research Field

Signal Transduction

Synonyms

BP180; BPA 2; BPAG2; COL17A1; Collagen 17; CollagenXVII; LAD1

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Collagen XVII alpha 1 Antibody (YA2759) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

Country or Region *

메일주소 *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Collagen XVII alpha 1 Antibody (YA2759)

Collagen XVII alpha 1 Antibody (YA2759) Featured Recommendations:

Product Detail

Verification Image

Background

제품 설명

Collagen XVII alpha 1 Antibody (YA2759) Related Classifications

최근 본 상품:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report