CD314/NKG2D Antibody (YA3918)
(Synonyms: KLRK1; KLR; NKG2D; NKG2-D; D12S2489E)CD314/NKG2D Antibody (YA3918) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD314/NKG2D.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
WB, FC, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
CD314/NKG2D Antibody (YA3918) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD314/NKG2D.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 25 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 25 kDa
Purified recombinant fragment of human CD314 (AA: extra 73-216) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD314/NKG2D functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4
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Subcellular Localization
Cell membrane; Single-pass type II membrane protein
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Expression
Tissue_specificity:It is expressed in natural killer (NK) cells, CD8 (+) αβ and γδ T cells. It is expressed on almost all CD56+CD3- NK cells in freshly isolated peripheral blood mononuclear cells (PBMCs) . It is also expressed in interferon-producing cytotoxic dendritic cells (IKDCs) .
Induction:Up-regulated by interleukin IL15 in primary NK cells -
Subunit
Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Does not interact with TYROBP. Interacts with CEACAM1; recruits PTPN6 that dephosphorylates VAV1 (PubMed:23696226)
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SwissProt ID
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Synonyms
KLRK1; KLR; NKG2D; NKG2-D; D12S2489E
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