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POT1 Antibody (YA1690)

(Synonyms: POT1; Protection of telomeres protein 1; hPot1; POT1-like telomere end-binding protein)
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Based on 1 Customer Validation

POT1 Antibody (YA1690) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to POT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • POT1 Antibody (YA1690) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

POT1 Antibody (YA1690) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to POT1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human POT1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for POT1 Antibody (YA1690)
    Western blot analysis of extracts from K562 (lane 2, 20ug), Hela (lane 3, 20ug), HepG2 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using POT1 Antibody (HY-P81945). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for POT1 Antibody (YA1690)
    Western blot analysis was performed on protein extracts (30 μg) from A431 (lane 2),HeLa (lane 3), 293T (lane 4),Ramos (lane 5),U87 (lane 6), and Jurkat (lane 7) using POT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    POT1 component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity

  • Subcellular Localization

    Nucleus; Chromosome, telomere

  • Expression


    Tissue_specificity:Ubiquitous

  • Isoforms & Post-Translational Modification

    Q9NUX5 has 2 isomers: Q9NUX5-1: 71442 Da (predicted); Q9NUX5-2: 60534 Da (predicted).

  • Subunit

    Homodimer or homooligomer (PubMed:17237768). Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP, ACD and POT1 (PubMed:15316005, PubMed:15383534). Binds single-stranded telomeric DNA as a monomer (PubMed:15558049). Associated component of the telomerase holoenzyme complex (PubMed:19179534). Found in a complex with TERF1, TINF2 and TNKS1 (PubMed:12768206). Interacts with TNKS1. Forms heterodimers with ACD (PubMed:15231715, PubMed:27013236, PubMed:35420632). Identified in a complex with ACD and single-stranded telomeric DNA (PubMed:17237768)

  • SwissProt ID

    Q9NUX5

  • Gene ID
    25913 [NCBI]
  • Synonyms

    POT1; Protection of telomeres protein 1; hPot1; POT1-like telomere end-binding protein

  • Research Field

    Epigenetics and Nuclear Signaling

POT1 Antibody (YA1690) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), Hela (lane 3, 20ug), HepG2 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using POT1 Antibody (HY-P81945). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from A431 (lane 2),HeLa (lane 3), 293T (lane 4),Ramos (lane 5),U87 (lane 6), and Jurkat (lane 7) using POT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • POT1 Antibody (YA1690) Product Image
    1/16
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • POT1 Antibody (YA1690) Product Image
  • Experimental Validation Results for POT1 Antibody (YA1690)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), Hela (lane 3, 20ug), HepG2 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using POT1 Antibody (HY-P81945). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for POT1 Antibody (YA1690)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from A431 (lane 2),HeLa (lane 3), 293T (lane 4),Ramos (lane 5),U87 (lane 6), and Jurkat (lane 7) using POT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • Experimental Validation Results for POT1 Antibody (YA1690)
  • POT1 Antibody (YA1690) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), Hela (lane 3, 20ug), HepG2 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using POT1 Antibody (HY-P81945). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • POT1 Antibody (YA1690) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from A431 (lane 2),HeLa (lane 3), 293T (lane 4),Ramos (lane 5),U87 (lane 6), and Jurkat (lane 7) using POT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • POT1 Antibody (YA1690) Product Image WB
  • POT1 Antibody (YA1690) Product Image WB