NKX3.1 Antibody (YA4452)

Cat. No.: HY-P84755: Data Sheet User Guide for Antibodies
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NKX3.1 Antibody (YA4452) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to NKX3.1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NKX3.1 Antibody (YA4452) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to NKX3.1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 26 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99801

Gene ID

4824 [NCBI]

Immunogen

Purified recombinant fragment of human NKX3A full(aa 1-234).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is highly expressed in the prostate and at a lower level in the testes.

Induction:By androgens and, in the LNCaP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen-independent phase

Isoforms & Post-Translational Modification：Q99801 has 5 isomers: Q99801-1: 26350 Da (predicted); Q99801-2: 21099 Da (predicted); Q99801-3: 18423 Da (predicted); Q99801-4: 19049 Da (predicted); Q99801-5: 21626 Da (predicted).
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues

Subunit：Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF (PubMed:11809674). Interacts with WDR77 (PubMed:12972618). Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation (PubMed:18077445). Interacts with FEM1B (By similarity)

Synonyms

NKX3; BAPX2; NKX3A; NKX3.1; NKX3-1

Documentation

User Guide for Antibodies (1077 KB)