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BMP-2 Antibody (YA6139)

(Synonyms: BMP2; BMP2A; Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A; BMP-2A)
Customer Review

Based on 1 Customer Validation

BMP-2 Antibody (YA6139) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BMP-2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • BMP-2 Antibody (YA6139) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

BMP-2 Antibody (YA6139) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BMP-2.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa,13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa,13 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719159

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Western blot analysis of extracts from Hela (lane2(20μg), SaoS-2 (lane3(20μg), HUVEC (lane4(20μg) and MCF-7 (lane5(20μg) using BMP-2 Antibody (HY-P86447). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunocytochemistry analysis of MCF-7 cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    Immunocytochemistry analysis of Hela cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    BMP-2 is a Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is particularly abundant in the lungs, spleen, and colon, and present in lower but significant amounts in the heart, brain, placenta, liver, skeletal muscle, kidneys, pancreas, prostate, ovaries, and small intestine.

  • Subunit

    Homodimer; disulfide-linked (PubMed:10074410). Interacts with SOSTDC1 (PubMed:15020244). Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN (By similarity). Interacts with MAFP5 (By similarity). Interacts with FBN1 (via N-terminal domain) and FBN2 (PubMed:18339631). Interacts with type I receptor BMPR1A (PubMed:15064755). Interacts with type II receptor BMPR2 (PubMed:7791754). Interacts with SCUBE3 (PubMed:33308444). Interacts with TNFAIP6 (primarily via Link domain); this interaction is inhibited by hyaluronan. Interacts with ERFE (PubMed:31800957). Interacts with BMPR1A/ALK3; the interaction may induce HAMP expression (PubMed:31800957). Forms heterodimers with BMP6 in vitro; the heterodimer then binds to its receptor BMPR1A /ALK3 and may induce HAMP expression (PubMed:31800957). Interacts with TGFBR3 (PubMed:18184661)

  • SwissProt ID

    P12643

  • Gene ID
    650 [NCBI]
  • Synonyms

    BMP2; BMP2A; Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A; BMP-2A

BMP-2 Antibody (YA6139) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), SaoS-2 (lane3(20μg), HUVEC (lane4(20μg) and MCF-7 (lane5(20μg) using BMP-2 Antibody (HY-P86447). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • BMP-2 Antibody (YA6139) Product Image
    1/16
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • BMP-2 Antibody (YA6139) Product Image
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), SaoS-2 (lane3(20μg), HUVEC (lane4(20μg) and MCF-7 (lane5(20μg) using BMP-2 Antibody (HY-P86447). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • Experimental Validation Results for BMP-2 Antibody (YA6139)
  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), SaoS-2 (lane3(20μg), HUVEC (lane4(20μg) and MCF-7 (lane5(20μg) using BMP-2 Antibody (HY-P86447). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using BMP-2 Antibody (YA6139). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86447, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • BMP-2 Antibody (YA6139) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling BMP-2 with BMP-2 Antibody (HY-P86447) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with BMP-2 Antibody (HY-P86447) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • BMP-2 Antibody (YA6139) Product Image WB
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image IHC-P
  • BMP-2 Antibody (YA6139) Product Image ICC
  • BMP-2 Antibody (YA6139) Product Image ICC