TCF-4/TCF7L2 Antibody (YA2167)(PBS only)
(Synonyms: TCF7L2; HMG box transcription factor 4; HTCF-4; T-cell factor-4 variant X2; T-cell factor 4; Transcription factor 7-like 2; TCF-4)TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
A synthetic peptide of human TCF-4 aa2-14.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TCF-4/TCF7L2 participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine
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Subcellular Localization
Nucleus, PML body; Nucleus
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Expression
Tissue_specificity:This gene was detected in small intestinal epithelial cells, with the highest expression at the top of the crypts and a gradient of expression from the crypts to the villi. It was also detected in colonic epithelial cells and colon cancer, as well as in breast epithelial cells and their derivative carcinomas. -
Isoforms & Post-Translational Modification
Q9NQB0 has 17 isomers: Q9NQB0-1: 67919 Da (predicted); Q9NQB0-2: 55152 Da (predicted); Q9NQB0-3: 67976 Da (predicted); Q9NQB0-4: 53270 Da (predicted); Q9NQB0-5: 66037 Da (predicted); Q9NQB0-6: 53676 Da (predicted); Q9NQB0-7: 66094 Da (predicted); Q9NQB0-8: 65291 Da (predicted); Q9NQB0-9: 51794 Da (predicted); Q9NQB0-10: 50611 Da (predicted); Q9NQB0-11: 54572 Da (predicted); Q9NQB0-12: 51159 Da (predicted); Q9NQB0-13: 63466 Da (predicted); Q9NQB0-14: 49166 Da (predicted); Q9NQB0-15: 49688 Da (predicted); Q9NQB0-16: 63930 Da (predicted); Q9NQB0-17: 36884 Da (predicted).
In vitro, phosphorylated by TNIK;Phosphorylated at Thr-201 and/or Thr-212 by NLK. Phosphorylation by NLK at these sites inhibits DNA-binding by TCF7L2/TCF4, thereby preventing transcriptional activation of target genes of the canonical Wnt/beta-catenin signaling pathway;Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylation is enhanced by SENP2. Sumoylation/desumoylation regulates TCF7L2/TCF4 transcription activity in the Wnt/beta-catenin signaling pathway without altering interaction with CTNNB1 nor binding to DNA -
Subunit
Interacts with TGFB1I1 (By similarity). Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex (PubMed:10080941, PubMed:17052462, PubMed:19816403, PubMed:28829046, PubMed:29739711, PubMed:9065401, PubMed:9916915). Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Interacts with DDIT3/CHOP. The CTNNB1 and TCF7L2/TCF4 complex interacts with PML (isoform PML-4). Identified in a complex with CTNNB1 and FERMT2. Interacts with SPIN1 (PubMed:22258766, PubMed:24589551, PubMed:29061846). Interacts with C11orf84/SPINDOC in a SPIN1-dependent manner (PubMed:29061846). Interacts with DAZAP2; the interaction results in localization of DAZAP2 to the nucleus (PubMed:19304756)
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SwissProt ID
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Synonyms
TCF7L2; HMG box transcription factor 4; HTCF-4; T-cell factor-4 variant X2; T-cell factor 4; Transcription factor 7-like 2; TCF-4
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Research Field
Epigenetics and Nuclear Signaling
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