TCF-4/TCF7L2 Antibody (YA2167)(PBS only)

Cat. No.: HY-P82422A: Ficha de datos User Guide for Antibodies
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TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

Host

Rabbit

Peso molecular

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NQB0

Gene ID

6934 [NCBI]

Immunogen

A synthetic peptide of human TCF-4 aa2-14.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Pureza	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine

Subcellular Localization：Nucleus, PML body; Nucleus

Expression：
Tissue_specificity:This gene was detected in small intestinal epithelial cells, with the highest expression at the top of the crypts and a gradient of expression from the crypts to the villi. It was also detected in colonic epithelial cells and colon cancer, as well as in breast epithelial cells and their derivative carcinomas.

Isoforms & Post-Translational Modification：Q9NQB0 has 17 isomers: Q9NQB0-1: 67919 Da (predicted); Q9NQB0-2: 55152 Da (predicted); Q9NQB0-3: 67976 Da (predicted); Q9NQB0-4: 53270 Da (predicted); Q9NQB0-5: 66037 Da (predicted); Q9NQB0-6: 53676 Da (predicted); Q9NQB0-7: 66094 Da (predicted); Q9NQB0-8: 65291 Da (predicted); Q9NQB0-9: 51794 Da (predicted); Q9NQB0-10: 50611 Da (predicted); Q9NQB0-11: 54572 Da (predicted); Q9NQB0-12: 51159 Da (predicted); Q9NQB0-13: 63466 Da (predicted); Q9NQB0-14: 49166 Da (predicted); Q9NQB0-15: 49688 Da (predicted); Q9NQB0-16: 63930 Da (predicted); Q9NQB0-17: 36884 Da (predicted).
In vitro, phosphorylated by TNIK;Phosphorylated at Thr-201 and/or Thr-212 by NLK. Phosphorylation by NLK at these sites inhibits DNA-binding by TCF7L2/TCF4, thereby preventing transcriptional activation of target genes of the canonical Wnt/beta-catenin signaling pathway;Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylation is enhanced by SENP2. Sumoylation/desumoylation regulates TCF7L2/TCF4 transcription activity in the Wnt/beta-catenin signaling pathway without altering interaction with CTNNB1 nor binding to DNA

Subunit：Interacts with TGFB1I1 (By similarity). Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex (PubMed:10080941, PubMed:17052462, PubMed:19816403, PubMed:28829046, PubMed:29739711, PubMed:9065401, PubMed:9916915). Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Interacts with DDIT3/CHOP. The CTNNB1 and TCF7L2/TCF4 complex interacts with PML (isoform PML-4). Identified in a complex with CTNNB1 and FERMT2. Interacts with SPIN1 (PubMed:22258766, PubMed:24589551, PubMed:29061846). Interacts with C11orf84/SPINDOC in a SPIN1-dependent manner (PubMed:29061846). Interacts with DAZAP2; the interaction results in localization of DAZAP2 to the nucleus (PubMed:19304756)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

TCF7L2; HMG box transcription factor 4; HTCF-4; T-cell factor-4 variant X2; T-cell factor 4; Transcription factor 7-like 2; TCF-4

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/sw480-cell.html [Content Brief]