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TROP2 Antibody (YA1496)

(Synonyms: TACSTD2; GA733-1; M1S1; TROP2; Tumor-associated calcium signal transducer 2; Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; Pancreatic carcinoma marker protein GA733-1)
Customer Review

Based on 1 Customer Validation

TROP2 Antibody (YA1496) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TROP2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • TROP2 Antibody (YA1496) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:100-1:200 1:50-1:100 1:10000

Product Details

Description

TROP2 Antibody (YA1496) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TROP2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45-65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database

Entrez Gene: 4070 Human

SwissProt: P09758 Human

Immunogen

Purified recombinant fragment of human TACSTD2(AA: Extra 27-274) expressed in E. Coli.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Western blot analysis of extracts from HCT116(lane 2(20μg) , SK-BR-3(lane 3(20μg) ,PC-3(lane 4(20μg)and A431( lane 5(20μg) using TROP2 Antibody (HY-P81751). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    TROP2 is a transmembrane glycoprotein that belongs to the GA733 family. Trop2 has several ligands, including claudin-1, claudin-7, and cyclin D1. Trop2 activity requires regulated intramembrane proteolysis (RIP) . RIP cleaves Trop2 by TNF-α converting enzyme (TACE), followed by γ-secretase cleavage within the transmembrane domain. TROP2 can activate the ERK1/3-MAPK pathway. TROP2 signals cells to self-renewal, proliferation, invasion, and survival. Trop2 has stem cell-like properties. TROP2 is overexpressed in a variety of human cancers, including breast, cervical, colorectal, esophageal (some types), lung (some types), non-Hodgkin lymphoma, chronic lymphocytic lymphoma (CLL), Rajburkitt's lymphoma, oral squamous cell carcinoma, ovarian, pancreatic, prostate, gastric, thyroid, bladder, and uterine cancers.

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Placental and pancreatic cancer cell lines

  • SwissProt ID

    P09758

  • Gene ID
    4070 [NCBI]
  • Synonyms

    TACSTD2; GA733-1; M1S1; TROP2; Tumor-associated calcium signal transducer 2; Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; Pancreatic carcinoma marker protein GA733-1

  • Research Field

    Cancer

References

[1]. Shvartsur A, et al. Trop2 and its overexpression in cancers: regulation and clinical/therapeutic implications. Genes Cancer. 2015 Mar;6(3-4):84-105. [Content Brief]

TROP2 Antibody (YA1496) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20μg) , SK-BR-3(lane 3(20μg) ,PC-3(lane 4(20μg)and A431( lane 5(20μg) using TROP2 Antibody (HY-P81751). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    1/16
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • TROP2 Antibody (YA1496) Product Image
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20μg) , SK-BR-3(lane 3(20μg) ,PC-3(lane 4(20μg)and A431( lane 5(20μg) using TROP2 Antibody (HY-P81751). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • Experimental Validation Results for TROP2 Antibody (YA1496)
  • TROP2 Antibody (YA1496) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116(lane 2(20μg) , SK-BR-3(lane 3(20μg) ,PC-3(lane 4(20μg)and A431( lane 5(20μg) using TROP2 Antibody (HY-P81751). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TROP2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81751, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma‌ tissue using TROP2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81751, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TROP2 Antibody (YA1496) Product Image WB
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image IHC-P
  • TROP2 Antibody (YA1496) Product Image mIHC
  • TROP2 Antibody (YA1496) Product Image mIHC
  • TROP2 Antibody (YA1496) Product Image mIHC
  • TROP2 Antibody (YA1496) Product Image mIHC
  • TROP2 Antibody (YA1496) Product Image mIHC
  • TROP2 Antibody (YA1496) Product Image mIHC