G3BP2 Antibody
(Synonyms: KIAA0660, G3BP2, Ras GTPase-activating protein-binding protein 2, G3BP-2, GAP SH3 domain-binding protein 2)G3BP2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to G3BP2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
G3BP2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to G3BP2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 54; 51 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 54 kDa
Synthetic peptide corresponding to the C-term region of human G3BP2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
G3BP2 is a Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling. Plays an essential role in stress granule formation. Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress. Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Cytoplasm, Stress granule
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Isoforms & Post-Translational Modification
G3BP2 has 2 isoforms, Q9UN86-1: amino acid length is 482, molecular weight is 54121 Da (predicted); Q9UN86-2: amino acid length is 449, molecular weight is 50817 Da (predicted).
(Microbial infection) Cleaved by foot-and-mouth disease virus leader protease; this cleavage suppresses the formation of cytoplasmic stress granules. -
Subunit
Forms homooligomers.
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SwissProt ID
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Synonyms
KIAA0660, G3BP2, Ras GTPase-activating protein-binding protein 2, G3BP-2, GAP SH3 domain-binding protein 2
Documentation
[1]. Matsuki H, et al. Both G3BP1 and G3BP2 contribute to stress granule formation. Genes Cells. 2013 Feb;18(2):135-46. [Content Brief]
[2]. Sanders DW, et al. Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Cell. 2020 Apr 16;181(2):306-324.e28. [Content Brief]
[3]. Yang P, et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell. 2020 Apr 16;181(2):325-345.e28. [Content Brief]
[4]. Guillén-Boixet J, et al. RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation. Cell. 2020 Apr 16;181(2):346-361.e17. [Content Brief]
[5]. Kedersha N, et al. G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits. J Cell Biol. 2016 Mar 28;212(7):845-60. [Content Brief]
[6]. Jia X, et al. De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders. Sci Adv. 2022 Aug 19;8(33):eabo7112. [Content Brief]
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