CD82 Antibody (YA3925)

製品番号: HY-P84228: データシート SDS COA User Guide for Antibodies
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CD82 Antibody (YA3925) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD82.

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容量	価格（税別）	在庫状況	数量
20 μL	$167	在庫あり	Estimated Time of Arrival: December 31
50 μL	$252	在庫あり	Estimated Time of Arrival: December 31
100 μL	$408	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

CD82 Antibody (YA3925) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD82.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 30 kDa;

Observed band size: 30-60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P27701

Gene ID

3732 [NCBI]

Immunogen

Purified recombinant fragment of human CD82 (AA: extra 111-228) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Flow cytometric analysis of 1X10^6 HT-29 cells labeling CD82 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:19497983). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization (PubMed:10985391, PubMed:35538033). Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC (PubMed:23897813). Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4 (PubMed:24623721, PubMed:8757325). Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1 (PubMed:15557282, PubMed:17560548). Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR (PubMed:15677461). Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly (By similarity). Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface (PubMed:36945827). Plays a role in the activation of macrophages into anti-inflammatory phenotypes (By similarity). Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation (By similarity). Also acts to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor (PubMed:34530889)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle, phagosome

Expression：
Tissue_specificity:Lymphatic specific

Isoforms & Post-Translational Modification：P27701 has 2 isomers: P27701-1: 29626 Da (predicted); P27701-2: 26818 Da (predicted).
Palmitoylated. Palmitoylation contributes to oligomerization and surface expression

Subunit：Forms homooligomers (PubMed:24623721). Interacts directly with IGSF8 (PubMed:12750295). Interacts with EGFR (PubMed:10985391, PubMed:35538033). Interacts with VEGFA and PDGFB (PubMed:34530889). Interacts with ITGA4 (PubMed:8757325). Interacts with ITGA6; this interaction reduces ITGA6 cell surface expression (PubMed:15557282). Interacts with ITGB1 (PubMed:8757325). Interacts with TLR4; this interaction inhibits TLR4-mediated signaling pathway (PubMed:36945827). Interacts with TLR9 (By similarity). Interacts with PLAUR (PubMed:15677461)

Synonyms

R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27

ドキュメンテーション

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データシート (233 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)