AAK1 Antibody
(Synonyms: KIAA1048, AAK1, AP2-associated protein kinase 1, Adaptor-associated kinase 1)AAK1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to AAK1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-100 |
Product Details
AAK1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to AAK1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 142 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 103 kDa
Synthetic peptide corresponding to the center region of human AAK1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
AAK1 regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cell membrane; Membrane, clathrin-coated pit; Presynapse
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Expression
Tissue_Specificity: Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. -
Isoforms & Post-Translational Modification
AAK1 has 2 isoforms, Q2M2I8-1: amino acid length is 961, molecular weight is 103885 Da (predicted); Q2M2I8-2: amino acid length is 863, molecular weight is 93578 Da (predicted).Autophosphorylated
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Subunit
Interacts (via CBD domain) with clathrin.
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SwissProt ID
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Synonyms
KIAA1048, AAK1, AP2-associated protein kinase 1, Adaptor-associated kinase 1
Documentation
[1]. Ricotta D, et al. Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J Cell Biol. 2002 Mar 4;156(5):791-5. [Content Brief]
[2]. Conner SD, et al. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J Cell Biol. 2002 Mar 4;156(5):921-9. [Content Brief]
[3]. Conner SD, et al. Differential requirements for AP-2 in clathrin-mediated endocytosis. J Cell Biol. 2003 Sep 1;162(5):773-9. [Content Brief]
[4]. Conner SD, et al. AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin. Traffic. 2003 Dec;4(12):885-90. [Content Brief]
[5]. Henderson DM, et al. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol Biol Cell. 2007 Jul;18(7):2698-706. [Content Brief]
[6]. Neveu G, et al. AP-2-associated protein kinase 1 and cyclin G-associated kinase regulate hepatitis C virus entry and are potential drug targets. J Virol. 2015 Apr;89(8):4387-404. [Content Brief]
[7]. Sorensen EB, et al. AAK1 regulates Numb function at an early step in clathrin-mediated endocytosis. Traffic. 2008 Sep;9(10):1791-800. [Content Brief]
[8]. Gupta-Rossi N, et al. The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway. J Biol Chem. 2011 May 27;286(21):18720-30. [Content Brief]
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