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ACADM Antibody (YA1967)

(Synonyms: ACADM; Medium-chain specific acyl-CoA dehydrogenase; mitochondrial; MCAD)
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Based on 1 Customer Validation

ACADM Antibody (YA1967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACADM Antibody (YA1967)
  • ACADM Antibody (YA1967) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ACADM Antibody (YA1967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADM.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ACADM

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104335

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACADM Antibody (YA1967)
    Western blot analysis of extracts from K562 (lane 2(20μg)), A431 (lane 3(20μg)), Rat heart (lane 4(20μg)) and Mouse liver(lane 5(20μg)) using ACADM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ACADM is a Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains[1][2][3][4][5][6].

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    P11310 has 2 isomers: P11310-1: 46588 Da (predicted); P11310-2: 47020 Da (predicted).
    Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3

  • Subunit

    Homotetramer (PubMed:8823176, Ref.23). Interacts with the heterodimeric electron transfer flavoprotein ETF

  • SwissProt ID

    P11310

  • Gene ID
    34 [NCBI]
  • Synonyms

    ACADM; Medium-chain specific acyl-CoA dehydrogenase; mitochondrial; MCAD

  • Research Field

    Signal Transduction

ACADM Antibody (YA1967) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg)), A431 (lane 3(20μg)), Rat heart (lane 4(20μg)) and Mouse liver(lane 5(20μg)) using ACADM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACADM Antibody (YA1967) Product Image
    1/16
  • Experimental Validation Results for ACADM Antibody (YA1967)
  • ACADM Antibody (YA1967) Product Image
  • Experimental Validation Results for ACADM Antibody (YA1967)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg)), A431 (lane 3(20μg)), Rat heart (lane 4(20μg)) and Mouse liver(lane 5(20μg)) using ACADM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ACADM Antibody (YA1967)
  • ACADM Antibody (YA1967) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg)), A431 (lane 3(20μg)), Rat heart (lane 4(20μg)) and Mouse liver(lane 5(20μg)) using ACADM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACADM Antibody (YA1967) Product Image WB