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F4/80 Antibody (YA920)

(Synonyms: F4/80 (mouse); Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; AGRE1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.)
Customer Review

Based on 1 Customer Validation

F4/80 Antibody (YA920) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to F4/80.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • F4/80 Antibody (YA920) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:1000 1:200-1:1000 1:50-1:100 1:50-1:500 1:200-1:1000

Product Details

Description

F4/80 Antibody (YA920) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to F4/80.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130-180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 102 kDa
Species Reactivity Database
Immunogen

Recombinant protein within mouse F4/80 aa 1-650 / 931.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103198

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for F4/80 Antibody (YA920)
    Western blot analysis of extracts from RAW264.7(lane 2(20μg), RAW264.7(lane 3(40μg) using ADGRE1 (F4/80)(HY-P81083) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for F4/80 Antibody (YA920)
    Immunocytochemistry analysis of RAW264.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for F4/80 Antibody (YA920)
    Immunocytochemistry analysis of RAW64.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for F4/80 Antibody (YA920)
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for F4/80 Antibody (YA920)
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    F4/80 is an Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is expressed in macrophages; however, it is not expressed in macrophages in the T-cell regions of lymph nodes and spleen. Expression levels are low on blood monocytes.
    Positive sample: RAW264.7 cell lysate, rat spleen tissue lysate, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue.

  • SwissProt ID

    Q61549 Mouse

  • Gene ID
    13733 [NCBI]
  • Synonyms

    F4/80 (mouse); Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; AGRE1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.

F4/80 Antibody (YA920) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg), RAW264.7(lane 3(40μg) using ADGRE1 (F4/80)(HY-P81083) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW264.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW64.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • F4/80 Antibody (YA920) Product Image
    1/16
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • F4/80 Antibody (YA920) Product Image
  • Experimental Validation Results for F4/80 Antibody (YA920)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg), RAW264.7(lane 3(40μg) using ADGRE1 (F4/80)(HY-P81083) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for F4/80 Antibody (YA920)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW264.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for F4/80 Antibody (YA920)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW64.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for F4/80 Antibody (YA920)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for F4/80 Antibody (YA920)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • Experimental Validation Results for F4/80 Antibody (YA920)
  • F4/80 Antibody (YA920) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20μg), RAW264.7(lane 3(40μg) using ADGRE1 (F4/80)(HY-P81083) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • F4/80 Antibody (YA920) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW264.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • F4/80 Antibody (YA920) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW64.7 cells labeling ADGRE1 (F4/80) with ADGRE1 (F4/80) Antibody (HY-P81083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADGRE1 (F4/80) Antibody (HY-P81083)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • F4/80 Antibody (YA920) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • F4/80 Antibody (YA920) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ADGRE1 (F4/80) Antibody (YA920). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81083, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • F4/80 Antibody (YA920) Product Image WB
  • F4/80 Antibody (YA920) Product Image ICC
  • F4/80 Antibody (YA920) Product Image ICC
  • F4/80 Antibody (YA920) Product Image IHC-P
  • F4/80 Antibody (YA920) Product Image IHC-P