Cofilin Antibody (YA5414)

Cat. No.: HY-P85722: Data Sheet User Guide for Antibodies
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Cofilin Antibody (YA5414) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cofilin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cofilin Antibody (YA5414) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cofilin.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 19 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P23528

Gene ID

1072 [NCBI]

Immunogen

Synthesized peptide derived from human Cofilin

Application &
Dilution Ratio

Application	Dilution Ratio
IHC1	50-200
WB WB: Western Blot	1:1000-2000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity)

Subcellular Localization：Nucleus matrix; Cytoplasm, cytoskeleton; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium; Cell projection, growth cone; Cell projection, axon

Expression：
Tissue_specificity:Widely distributed in various tissues

Induction:Up-regulated in response to enterovirus 71 (EV71) infection (at protein level)

Subunit：Can bind G- and F-actin in a 1:1 ratio of cofilin to actin (PubMed:11812157). It is a major component of intranuclear and cytoplasmic actin rods (By similarity). Interacts with the subcortical maternal complex (SCMC) via interaction with TLE6 isoform 1 and NLRP5 (By similarity). Interacts with C9orf72 (By similarity)

Synonyms

Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform;

Documentation

User Guide for Antibodies (1077 KB)