ACADVL/VLCAD Antibody (YA6454)

Cat. No.: HY-P86761: Data Sheet COA
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ACADVL/VLCAD Antibody (YA6454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADVL/VLCAD.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ACADVL/VLCAD Antibody (YA6454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADVL/VLCAD.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P49748

Gene ID

37 [NCBI]

Immunogen

Recombinant protein within Human ACADVL / VLCAD aa 1-100 / 655.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431 (lane 1(20μg)) and HepG2 (lane 2(20μg)) using ACADVL/VLCAD Antibody (HY-P86761) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9599005, PubMed:9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed:21237683, PubMed:9839948)

Subcellular Localization：Mitochondrion inner membrane,Mitochondrion inner membrane

Expression：
Tissue_Specificity: Predominantly expressed in heart and skeletal muscle (at protein level). Also detected in kidney and liver (at protein level)

Isoforms & Post-Translational Modification：P49748 has three isomers: P49748-1: 70390 Da (predicted); P49748-2: 68058 Da (predicted); P49748-3: 72927 Da (predicted).
S-nitrosylation at Cys-237 in liver improves catalytic efficiency

Subunit：Homodimer (PubMed:17374501, PubMed:18227065, PubMed:9461620, PubMed:9599005)

Synonyms

ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; Very long-chain specific acyl-CoA dehydrogenase antibody; VLCAD antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)