ACADVL/VLCAD Antibody (YA6454)
(Synonyms: ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; Very long-chain specific acyl-CoA dehydrogenase antibody; VLCAD antibody; )
Based on 1 Customer Validation
ACADVL/VLCAD Antibody (YA6454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADVL/VLCAD.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IF-Tissue, IP
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Reactivity :
Human
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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| Dilution Ratio | 1:1000 | 1:1000 | 1:100 | 1:200 | 1-2μg/sample |
Product Details
ACADVL/VLCAD Antibody (YA6454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACADVL/VLCAD.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 70 kDa
Recombinant protein within Human ACADVL / VLCAD aa 1-100 / 655.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A431 (lane 1(20μg)) and HepG2 (lane 2(20μg)) using ACADVL/VLCAD Antibody (HY-P86761) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skin tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ACADVL/VLCAD Antibody (HY-P86761, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
ACADVL/VLCAD is a Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains[1][2][3][4][5][6].
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Subcellular Localization
Mitochondrion inner membrane,Mitochondrion inner membrane
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Expression
Tissue_Specificity: Predominantly expressed in heart and skeletal muscle (at protein level). Also detected in kidney and liver (at protein level) -
Isoforms & Post-Translational Modification
P49748 has three isomers: P49748-1: 70390 Da (predicted); P49748-2: 68058 Da (predicted); P49748-3: 72927 Da (predicted).
S-nitrosylation at Cys-237 in liver improves catalytic efficiency -
Subunit
Homodimer (PubMed:17374501, PubMed:18227065, PubMed:9461620, PubMed:9599005)
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SwissProt ID
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Synonyms
ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; ACAD 6 antibody; ACAD6 antibody; ACADV_HUMAN antibody; Acadvl antibody; Acyl CoA dehydrogenase very long chain antibody; Acyl Coenzyme A dehydrogenase very long chain antibody; LCACD antibody; mitochondrial antibody; Very long chain specific acyl CoA dehydrogenase antibody; Very long chain specific acyl CoA dehydrogenase mitochondrial antibody; Very long-chain specific acyl-CoA dehydrogenase antibody; VLCAD antibody;
Documentation
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