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Phospho-Vimentin (Ser56) Antibody (YA138)

(Synonyms: VIM; Vimentin)
Customer Review

Based on 1 Customer Validation

Phospho-Vimentin (Ser56) Antibody (YA138) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Vimentin (Ser56).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Phospho-Vimentin (Ser56) Antibody (YA138) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Vimentin (Ser56).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

Entrez Gene: 7431 Human

SwissProt: P08670 Human

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser56 of Human Vimentin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102732

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    Western blot analysis of extracts from U251(lane 2(20ug) and U251(lane 3(40ug)using Phospho-Vimentin Antibody (HY-P80863) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Vimentin is a Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Nucleus matrix; Cell membrane

  • Expression


    Tissue_specificity:It is highly expressed in fibroblasts, expressed in small amounts in T cells and B cells, and expressed very low or not at all in Burkitt lymphoma cell lines. It is expressed in many hormone-independent breast cancer cell lines.

    Induction:Up-regulated by muramyl-dipeptide and lipopolysaccharide

  • Subunit

    Homomer assembled from elementary dimers (PubMed:20176112). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with BCAS3 (PubMed:17505058). Interacts with LGSN (By similarity). Interacts with SYNM (By similarity). Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with PLEC isoform 1C (PubMed:24940650). Interacts with STK33 (PubMed:18811945). Interacts with LARP6 (PubMed:21746880). Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton (PubMed:16361107, PubMed:18827015). Interacts with TOR1AIP1 (PubMed:16361107). Interacts with DIAPH1 (PubMed:23325789). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132). Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM (PubMed:29496907). Interacts with NOD2 (PubMed:27812135). Interacts (via head region) with CORO1C (By similarity). Interacts with HDGF (isoform 2) (PubMed:26845719). Interacts with PRKCE (via phorbol-ester/DAG-type 2 domain) (PubMed:18408015). Interacts with BFSP2 (By similarity). Interacts with PPL (By similarity). Interacts (via rod domain) with PKP1 (PubMed:10852826). Interacts with PKP2 (PubMed:10852826). Interacts with SCRIB (via PDZ domains); the interaction protects SCRIB from proteasomal degradation and facilitates SCRIB localization to intermediate filaments, the interaction is reduced by cell contact inhibition (PubMed:19386766)

  • SwissProt ID

    P08670

  • Gene ID
    7431 [NCBI]
  • Synonyms

    VIM; Vimentin

  • Research Field

    Neuroscience

Phospho-Vimentin (Ser56) Antibody (YA138) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U251(lane 2(20ug) and U251(lane 3(40ug)using Phospho-Vimentin Antibody (HY-P80863) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
    1/16
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U251(lane 2(20ug) and U251(lane 3(40ug)using Phospho-Vimentin Antibody (HY-P80863) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Experimental Validation Results for Phospho-Vimentin (Ser56) Antibody (YA138)
  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U251(lane 2(20ug) and U251(lane 3(40ug)using Phospho-Vimentin Antibody (HY-P80863) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Vimentin (Ser56) with Phospho-Vimentin (Ser56) Antibody (HY-P80863) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image WB
  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image ICC
  • Phospho-Vimentin (Ser56) Antibody (YA138) Product Image ICC