eIF4A1 Antibody (YA5372)
(Synonyms: Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; )eIF4A1 Antibody (YA5372) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eIF4A1.
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Host:
Mouse
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:1000-3000 | 1:100-200 | 1:50-300 |
Product Details
eIF4A1 Antibody (YA5372) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eIF4A1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Synthetic Peptide of eIF4A1
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
eIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth[1].
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Subcellular Localization
Cytoplasm, perinuclear region; Cell membrane; Cytoplasm, Stress granule
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Isoforms & Post-Translational Modification
P60842 has 3 isomers: P60842-1: 46154 Da (predicted); P60842-2: 39548 Da (predicted); P60842-3: 22805 Da (predicted).
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Subunit
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner (PubMed:18596238). Interacts with PKP1 (via N-terminus); the interaction promotes EIF4A1 recruitment to the cap-dependent translation complex and EIF4A1 ATPase activity (PubMed:20156963, PubMed:23444369)
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SwissProt ID
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Synonyms
Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1;
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