eIF4A1 Antibody (YA5372)

Cat. No.: HY-P85680: Data Sheet User Guide for Antibodies
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eIF4A1 Antibody (YA5372) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eIF4A1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

eIF4A1 Antibody (YA5372) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eIF4A1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P60842

Gene ID

1973 [NCBI]

Immunogen

Synthetic Peptide of eIF4A1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-3000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-300

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome (PubMed:20156963). In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth (PubMed:20156963)

Subcellular Localization：Cytoplasm, perinuclear region; Cell membrane; Cytoplasm, Stress granule

Isoforms & Post-Translational Modification：P60842 has 3 isomers: P60842-1: 46154 Da (predicted); P60842-2: 39548 Da (predicted); P60842-3: 22805 Da (predicted).

Subunit：eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner (PubMed:18596238). Interacts with PKP1 (via N-terminus); the interaction promotes EIF4A1 recruitment to the cap-dependent translation complex and EIF4A1 ATPase activity (PubMed:20156963, PubMed:23444369)

Synonyms

Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1;

Documentation

User Guide for Antibodies (1077 KB)