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Torsin 1A Antibody (YA2935)

(Synonyms: TOR1A; DQ2; DYT1; TorsinA)
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Based on 1 Customer Validation

Torsin 1A Antibody (YA2935) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Torsin 1A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Torsin 1A Antibody (YA2935)
  • Torsin 1A Antibody (YA2935) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

Torsin 1A Antibody (YA2935) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Torsin 1A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Species Reactivity Database

Entrez Gene: 1861 Human

SwissProt: O14656 Human

Immunogen

A synthesized peptide derived from human Torsin A aa300-332.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Torsin 1A Antibody (YA2935)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), HepG2 (lane 3), 293T (lane 4), U87 (lane 5), HeLa (lane 6), and THP-1 (lane 7) using Torsin 1A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Torsin 1A is a Protein with chaperone functions important for the control of protein folding, processing, stability and localization as well as for the reduction of misfolded protein aggregates. Involved in the regulation of synaptic vesicle recycling, controls STON2 protein stability in collaboration with the COP9 signalosome complex (CSN). In the nucleus, may link the cytoskeleton with the nuclear envelope, this mechanism seems to be crucial for the control of nuclear polarity, cell movement and, specifically in neurons, nuclear envelope integrity. Participates in the cellular trafficking and may regulate the subcellular location of multipass membrane proteins such as the dopamine transporter SLC6A3, leading to the modulation of dopamine neurotransmission. In the endoplasmic reticulum, plays a role in the quality control of protein folding by increasing clearance of misfolded proteins such as SGCE variants or holding them in an intermediate state for proper refolding. May have a redundant function with TOR1B in non-neural tissues

  • Subcellular Localization

    Endoplasmic reticulum lumen; Nucleus membrane; Peripheral membrane protein; Cell projection, growth cone; Cytoplasmic vesicle membrane; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Widely expressed. Highest levels in kidney and liver. In the brain, high levels found in the dopaminergic neurons of the substantia nigra pars compacta, as well as in the neocortex, hippocampus and cerebellum. Also highly expressed in the spinal cord

  • Isoforms & Post-Translational Modification

    O14656 has 2 isomers: O14656-1: 37809 Da (predicted); O14656-2: 22135 Da (predicted).
    N-glycosylated

  • Subunit

    Homohexamer. Interacts with TOR1B; the interaction may be specific of neural tissues. Interacts (ATP-bound) with TOR1AIP1 and TOR1AIP2; the interactions induce ATPase activity. Interacts with KLHL14; preferentially when ATP-free. Interacts with KLC1 (via TPR repeats); the interaction associates TOR1A with the kinesin oligomeric complex. Interacts with COPS4; the interaction associates TOR1A with the CSN complex. Interacts with SNAPIN; the interaction is direct and associates SNAPIN with the CSN complex. Interacts with STON2. Interacts (ATP-bound) with SYNE3 (via KASH domain); the interaction is required for SYNE3 nuclear envelope localization. Interacts with VIM; the interaction associates TOR1A with the cytoskeleton. Interacts with PLEC. Interacts (ATP-bound) with SLC6A3; regulates SLC6A3 transport to the plasma membrane

  • SwissProt ID

    O14656

  • Gene ID
    1861 [NCBI]
  • Synonyms

    TOR1A; DQ2; DYT1; TorsinA

  • Research Field

    Neuroscience

Torsin 1A Antibody (YA2935) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), HepG2 (lane 3), 293T (lane 4), U87 (lane 5), HeLa (lane 6), and THP-1 (lane 7) using Torsin 1A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Torsin 1A Antibody (YA2935) Product Image
    1/16
  • Experimental Validation Results for Torsin 1A Antibody (YA2935)
  • Torsin 1A Antibody (YA2935) Product Image
  • Experimental Validation Results for Torsin 1A Antibody (YA2935)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), HepG2 (lane 3), 293T (lane 4), U87 (lane 5), HeLa (lane 6), and THP-1 (lane 7) using Torsin 1A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Torsin 1A Antibody (YA2935)
  • Torsin 1A Antibody (YA2935) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), HepG2 (lane 3), 293T (lane 4), U87 (lane 5), HeLa (lane 6), and THP-1 (lane 7) using Torsin 1A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Torsin 1A Antibody (YA2935) Product Image WB