BACE Antibody (YA5112)
(Synonyms: BACE1; BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2)BACE Antibody (YA5112) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BACE.
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Host:
Mouse
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Application:
WB
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Reactivity :
Human, Rat, Bovine, Dog, Pig
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000-1:2000 |
Product Details
BACE Antibody (YA5112) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BACE.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat, Bovine, Dog, Pig
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Calculated Molecular Weight Predicted band size: 56 kDa;
Purified recombinant human BACE (C-terminus) protein fragments expressed in E.coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BACE is responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1[1][2][3].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Golgi apparatus, trans-Golgi network; Endoplasmic reticulum; Endosome; Cell surface; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane raft; Lysosome; Late endosome; Early endosome; Recycling endosome; Cell projection, axon; Cell projection, dendrite
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Expression
Tissue_specificity:It is highly expressed in the brain and pancreas. In the brain, the highest expression levels are found in the substantia nigra, locus coeruleus, and medulla oblongata.
Induction:Up-regulated by the Ca (2+) -regulated transcription factor NFATC4 -
Isoforms & Post-Translational Modification
P56817 has 6 isomers: P56817-1: 55764 Da (predicted); P56817-2: 52908 Da (predicted); P56817-3: 51068 Da (predicted); P56817-4: 48212 Da (predicted); P56817-5: 45017 Da (predicted); P56817-6: 42160 Da (predicted).
N-Glycosylated (PubMed:11083922, PubMed:17425515). Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions (By similarity);Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein;Palmitoylation mediates lipid raft localization;Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation (PubMed:16033761, PubMed:20484053, PubMed:23109336, PubMed:27302062). Monoubiquitinated and 'Lys-63'-linked polyubitinated (PubMed:20484053). Deubiquitnated by USP8; inhibits lysosomal degradation (PubMed:27302062);Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface -
Subunit
Monomer. Interacts (via DXXLL motif) with GGA1, GGA2 and GGA3 (via their VHS domain); the interaction highly increases when BACE1 is phosphorylated at Ser-498 (PubMed:14567678, PubMed:15886016). Interacts with RTN1; RTN2; RTN3 and RTN4; the interaction leads to inhibition of amyloid precursor protein processing (PubMed:15286784, PubMed:16965550, PubMed:16979658). Interacts with SNX6 (PubMed:20354142). Interacts with PCSK9 (PubMed:18660751). Interacts with NAT8 and NAT8B (PubMed:19011241). Interacts with BIN1 (PubMed:27179792). Interacts (via extracellular domain) with ADAM10 (via extracellular domain) (By similarity). Interacts with SORL1; this interaction may affect binding with APP and hence reduce APP cleavage (PubMed:16407538). Interacts with NRDC AND NRG1 (By similarity)
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SwissProt ID
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Synonyms
BACE1; BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2
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