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KLF15 Antibody (YA4561)

(Synonyms: KKLF; DKFZp779M1320)
Customer Review

Based on 1 Customer Validation

KLF15 Antibody (YA4561) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF15.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • KLF15 Antibody (YA4561) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

KLF15 Antibody (YA4561) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF15.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Immunogen

Purified recombinant fragment of human KLF15 aa 1-416.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KLF15 Antibody (YA4561)
    Western blot analysis of extracts from SMMC-7721(lane 1(40μg) ),Jukat(lane 2(40μg) ),293T(lane 3(40μg) ),MCF-7(lane 4(40μg) )and Hela(lane 5(40μg) ) using KLF15 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KLF15 Antibody (YA4561)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), HepG2 (lane 4), SH-SY5Y (lane 5), and K562 (lane 6) using KLF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KLF15 Antibody (YA4561)
    Flow cytometric analysis of 1X10^6 Ramos cells labeling KLF15 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    KLF15 transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4. Involved in podocyte differentiation. Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the liver, skeletal muscle, and kidneys. It is also expressed in cardiomyocytes. Expression is significantly reduced in cardiac tissues with non-ischemic cardiomyopathy and aortic aneurysms, as well as in glomerular diseases. It is not expressed in bone marrow or lymphoid tissues.

    Induction:In podocytes, up-regulated by retinoic acid

  • Subunit

    Interacts with MYOCD and EP300

  • SwissProt ID

    Q9UIH9

  • Gene ID
    28999 [NCBI]
  • Synonyms

    KKLF; DKFZp779M1320

KLF15 Antibody (YA4561) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SMMC-7721(lane 1(40μg) ),Jukat(lane 2(40μg) ),293T(lane 3(40μg) ),MCF-7(lane 4(40μg) )and Hela(lane 5(40μg) ) using KLF15 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), HepG2 (lane 4), SH-SY5Y (lane 5), and K562 (lane 6) using KLF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling KLF15 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • KLF15 Antibody (YA4561) Product Image
    1/16
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • KLF15 Antibody (YA4561) Product Image
  • Experimental Validation Results for KLF15 Antibody (YA4561)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SMMC-7721(lane 1(40μg) ),Jukat(lane 2(40μg) ),293T(lane 3(40μg) ),MCF-7(lane 4(40μg) )and Hela(lane 5(40μg) ) using KLF15 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KLF15 Antibody (YA4561)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), HepG2 (lane 4), SH-SY5Y (lane 5), and K562 (lane 6) using KLF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KLF15 Antibody (YA4561)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling KLF15 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • Experimental Validation Results for KLF15 Antibody (YA4561)
  • KLF15 Antibody (YA4561) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SMMC-7721(lane 1(40μg) ),Jukat(lane 2(40μg) ),293T(lane 3(40μg) ),MCF-7(lane 4(40μg) )and Hela(lane 5(40μg) ) using KLF15 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • KLF15 Antibody (YA4561) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), HepG2 (lane 4), SH-SY5Y (lane 5), and K562 (lane 6) using KLF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KLF15 Antibody (YA4561) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Ramos cells labeling KLF15 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • KLF15 Antibody (YA4561) Product Image WB
  • KLF15 Antibody (YA4561) Product Image WB
  • KLF15 Antibody (YA4561) Product Image FC