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KCNQ1 Antibody (YA4075)

(Synonyms: LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1; FLJ26167)
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Based on 1 Customer Validation

KCNQ1 Antibody (YA4075) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KCNQ1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KCNQ1 Antibody (YA4075)
  • KCNQ1 Antibody (YA4075) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

KCNQ1 Antibody (YA4075) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KCNQ1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Immunogen

Purified recombinant fragment of human KCNQ1 aa 229-347.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3718895

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KCNQ1 Antibody (YA4075)
    Western blot analysis of extracts from MCF-7(lane 2(20μg) , Raji (lane 3(20μg) ,Jurkat(lane 4(20μg)and U251( lane 5(20μg) using CARM1 Antibody（HY-P84378）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/750) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    KCNQ1 is a Pore-forming subunit of the voltage-gated potassium (Kv) channel involved in the regulation of cardiomyocyte excitability and important in normal development and functions of myocardium, inner ear, stomach and colon. Associates with KCNE beta subunits that modulates current kinetics. Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current. Also promotes a delayed voltage activated potassium current showing outward rectification characteristic. During beta-adrenergic receptor stimulation, participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current. When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions. This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents. During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion. Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion. When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current. When associated with KCNE4, inhibits voltage-gated potassium channel activity. When associated with KCNE5, this complex only conducts current upon strong and continued depolarization. Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity. Binds with phosphatidylinositol 4,5-bisphosphate. KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability; Non-functional alone but modulatory when coexpressed with the full-length isoform 1[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Early endosome; Membrane raft; Endoplasmic reticulum; Basolateral cell membrane; Apical cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries

  • Isoforms & Post-Translational Modification

    P51787 has 2 isomers: P51787-1: 74699 Da (predicted); P51787-2: 61474 Da (predicted).
    Phosphorylation at Ser-27 by PKA; increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9;Ubiquitinated by NEDD4L; promotes internalization (PubMed:22024150). The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A (PubMed:23529131);Deubiquitinated by USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization

  • Subunit

    Tetramer (PubMed:18165683, PubMed:19693805, PubMed:25441029, PubMed:31883792). Heterotetramer with KCNE1; targets to the membrane raft (PubMed:19693805, PubMed:20533308, PubMed:25037568). Interacts (via C-terminus) with calmodulin; forms a heterooctameric structure (with 4:4 KCNQ1:CALM stoichiometry); the interaction is calcium-independent, constitutive, participates in the proper assembly of a functional channel and also acts a calcium sensor (PubMed:16556865, PubMed:18165683, PubMed:25441029, PubMed:31883792). KCNQ1 channels interact more strongly with Ca(2+)-CALM than with apoCALM (PubMed:16556865). Interacts with AKAP9; targets protein kinase A (PKA) catalytic and regulatory subunits and protein phosphatase 1 (PP1) to the KCNQ1-KCNE1 complex, allowing PKA-mediated phosphorylation and increase of delayed rectifier potassium channel activity (PubMed:11799244, PubMed:25037568). Interacts with KCNE2; forms a heterooligomer complex that targets to the membrane raft and leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505, PubMed:20533308). Interacts with AP2M1; mediates estrogen-induced internalization via clathrin-coated vesicles (PubMed:23529131). Interacts with NEDD4L; promotes internalization and decreases I(Ks) currents (PubMed:22024150, PubMed:23529131). Interacts with USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restore plasma membrane localization (PubMed:22024150). Heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Interacts with KCNE3; four KCNE3 molecules are bound to one KCNQ1 tetramer (4:4 KCNQ1:KCNE3 stoichiometry); alters membrane raft localization; affects KCNQ1 structure and gating properties (PubMed:20533308, PubMed:31883792). Interacts with KCNE4; impairs KCNQ1 localization in lipid rafts and inhibits voltage-gated potassium channel activity (PubMed:19687231, PubMed:20533308). Interacts with KCNE5; impairs KCNQ1 localization in lipid rafts and only conducts current upon strong and continued depolarization (PubMed:12324418, PubMed:20533308). Interacts with SLC5A3; forms coregulatory channel-transporter complexes that modulate Na(+)-coupled myo-inositol influx through the transporter (By similarity)

  • SwissProt ID

    P51787

  • Gene ID
    3784 [NCBI]
  • Synonyms

    LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1; FLJ26167

KCNQ1 Antibody (YA4075) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , Raji (lane 3(20μg) ,Jurkat(lane 4(20μg)and U251( lane 5(20μg) using CARM1 Antibody（HY-P84378）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/750) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • KCNQ1 Antibody (YA4075) Product Image
    1/16
  • Experimental Validation Results for KCNQ1 Antibody (YA4075)
  • KCNQ1 Antibody (YA4075) Product Image
  • Experimental Validation Results for KCNQ1 Antibody (YA4075)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , Raji (lane 3(20μg) ,Jurkat(lane 4(20μg)and U251( lane 5(20μg) using CARM1 Antibody（HY-P84378）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/750) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KCNQ1 Antibody (YA4075)
  • KCNQ1 Antibody (YA4075) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg) , Raji (lane 3(20μg) ,Jurkat(lane 4(20μg)and U251( lane 5(20μg) using CARM1 Antibody（HY-P84378）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/750) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • KCNQ1 Antibody (YA4075) Product Image WB