Rab2 Antibody (YA5218)

Cat. No.: HY-P85526: Data Sheet User Guide for Antibodies
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Rab2 Antibody (YA5218) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Rab2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Rab2 Antibody (YA5218) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Rab2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P61019

Gene ID

5862 [NCBI]

Immunogen

Purified recombinant human Rab2 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (PubMed:37821429). RAB2A regulates autophagy by promoting autophagosome-lysosome fusion via recruitment of the HOPS endosomal tethering complex; this process involves autophagosomal RAB2A and lysosomal RAB39A recruitment of HOPS subcomplexes VPS39-VPS11 and VPS41-VPS16-VPS18-VPS33A, respectively, which assemble into a functional complex to mediate membrane tethering and SNAREs-driven membrane fusion (PubMed:37821429). Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi (PubMed:26209634). Together with RAB2B, redundantly required for efficient autophagic flux (PubMed:28483915)

Subcellular Localization：Endoplasmic reticulum-Golgi intermediate compartment membrane; Lipid-anchor; Cytoplasmic side; Melanosome; Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side; Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side; Cytoplasmic vesicle, secretory vesicle, acrosome; Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor; Cytoplasmic side

Isoforms & Post-Translational Modification：P61019 has 2 isomers: P61019-1: 23546 Da (predicted); P61019-2: 20847 Da (predicted).
Prenylated. Prenylation is required for association with cellular membranes

Subunit：Interacts with PRKCI (PubMed:14570876). Interacts with TRIP11 (PubMed:25473115).

Synonyms

9330148M11Rik; C80220; GTP binding protein; LHX; OTTMUSP00000004796; RAB2; RAB2 member RAS oncogene family; RAB2A; RAB2A, member RAS oncogene family; RAB2A_HUMAN; Ras related protein Rab 2A; RAS-associated protein RAB2; Ras-related protein Rab-2A; RP23-183L6.1; Small GTP binding protein RAB2A.

Documentation

User Guide for Antibodies (1077 KB)