BLK Antibody (YA4748)
(Synonyms: BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk)
Based on 1 Customer Validation
BLK Antibody (YA4748) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BLK.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:10000 |
Product Details
BLK Antibody (YA4748) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BLK.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 58 kDa;
Purified recombinant fragment of human Blk expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Blk, or B lymphoid kinase, is a Src family tyrosine kinase that shows B-lineage expression and tyrosine kinase activity, indicating a B-cell-restricted signaling role[1]. Mechanistically, Blk links to B cell receptor biology because, in a COS-cell reconstitution system, only Blk among Lyn, Blk, Hck, Syk, and Fyn phosphorylated and associated with Igα/Igβ chimeras[2]. In mice, Blk appears early in bone-marrow B-cell development and remains high in mature B cells, yet Blk-deficient mice showed unaltered B-cell development, activation, and humoral immune responses, supporting functional redundancy among Src family kinases[3]. In disease genetics, variants upstream of BLK associate with systemic lupus erythematosus, while autoimmune BLK haplotypes show reduced BLK expression and altered B-cell activation phenotypes in rheumatoid arthritis models[4][5]. Compared with related Src isoforms, Blk is distinguished by B-lineage enrichment, selective Igα/Igβ coupling in reconstituted BCR signaling, and redundancy rather than absolute requirement in mouse B-cell immunity[1][2][3]. For experimental applications, activated Blk undergoes E6AP-mediated ubiquitination and proteasomal degradation, and selective irreversible BLK inhibitors provide chemical tools for probing BLK-dependent signaling[6][7].
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Subcellular Localization
Cell membrane; Lipid-anchor
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Expression
Tissue_specificity:Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles
Induction:Expression is under the control of NF-kappa-B as well as the B-cell specific transcription factors PAX5 and EBF1 -
Subunit
Interacts with CBL (via SH2 domain). Interacts with CD79A and CD79B (via SH2 domain) (By similarity)
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SwissProt ID
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Synonyms
BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
Documentation
References
[1]. Dymecki SM, et al. Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells. Science. 1990 Jan 19;247(4940):332-6. [Content Brief]
[2]. Saouaf SJ, et al. Reconstitution of the B cell antigen receptor signaling components in COS cells. J Biol Chem. 1995 Nov 10;270(45):27072-8. [Content Brief]
[3]. Texido G, et al. The B-cell-specific Src-family kinase Blk is dispensable for B-cell development and activation. Mol Cell Biol. 2000 Feb;20(4):1227-33. [Content Brief]
[4]. Hom G, et al. Association of systemic lupus erythematosus with C8orf13-BLK and ITGAM-ITGAX. N Engl J Med. 2008 Feb 28;358(9):900-9. [Content Brief]
[5]. Simpfendorfer KR, et al. Autoimmune disease-associated haplotypes of BLK exhibit lowered thresholds for B cell activation and expansion of Ig class-switched B cells. Arthritis Rheumatol. 2015 Nov;67(11):2866-76. [Content Brief]
[6]. Oda H, et al. Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9557-62. [Content Brief]
[7]. Fu T, et al. Discovery of selective irreversible inhibitors of B-Lymphoid tyrosine kinase (BLK). Eur J Med Chem. 2022 Feb 5;229:114051. [Content Brief]
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