MTCO1 Antibody (YA6658)
(Synonyms: COI antibody; COX I antibody; COX1 antibody; COX1_HUMAN antibody; COXI antibody; Cytochrome c oxidase polypeptide I antibody; Cytochrome c oxidase subunit 1 antibody; Cytochrome C Oxidase subunit I antibody; Mitochondrially encoded cytochrome c oxidase I antibody; MT CO1 antibody; COI antibody; COX I antibody; COX1 antibody; COX1_HUMAN antibody; COXI antibody; Cytochrome c oxidase polypeptide I antibody; Cytochrome c oxidase subunit 1 antibody; Cytochrome C Oxidase subunit I antibody; Mitochondrially encoded cytochrome c oxidase I antibody; MT CO1 antibody; MT-CO1 antibody; MTCO 1 antibody; MTCO1 antibody; )Based on 1 Customer Validation
MTCO1 Antibody (YA6658) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTCO1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P, FC, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:50 | 1:10000 | 1:1000 | 1:500-1:2000 |
Product Details
MTCO1 Antibody (YA6658) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTCO1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 37 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 57 kDa
Recombinant protein within human MTCO1.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HeLa (lane 1(20μg)) and Neuro-2a (lane 2(20μg)) using MTCO1 Antibody (HY-P86965) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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Immunohistochemical analysis of paraffin-embedded human Prostate Cancer using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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Immunohistochemical analysis of paraffin-embedded human Bladder cancer using MTCO1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86965, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Background
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Function
MTCO1 component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix
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Subcellular Localization
Mitochondrion inner membrane
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Isoforms & Post-Translational Modification
P00395: 513 amino acids, molecular weight 57041 Da.
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Subunit
Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits
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SwissProt ID
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Synonyms
COI antibody; COX I antibody; COX1 antibody; COX1_HUMAN antibody; COXI antibody; Cytochrome c oxidase polypeptide I antibody; Cytochrome c oxidase subunit 1 antibody; Cytochrome C Oxidase subunit I antibody; Mitochondrially encoded cytochrome c oxidase I antibody; MT CO1 antibody; COI antibody; COX I antibody; COX1 antibody; COX1_HUMAN antibody; COXI antibody; Cytochrome c oxidase polypeptide I antibody; Cytochrome c oxidase subunit 1 antibody; Cytochrome C Oxidase subunit I antibody; Mitochondrially encoded cytochrome c oxidase I antibody; MT CO1 antibody; MT-CO1 antibody; MTCO 1 antibody; MTCO1 antibody;
Documentation
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Data Sheet (231 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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User Guide for Antibodies (1077 KB)