USP9X Antibody (YA1811)
(Synonyms: DFFRX; FAF; Fafl; Fam; hFAM; MRX99; Usp9x; X chromosome; X-linked)
Based on 1 Customer Validation
USP9X Antibody (YA1811) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP9X.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100-1:200 | 1:50-1:200 | 1:50-1:100 |
Product Details
USP9X Antibody (YA1811) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP9X.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 290 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 290 kDa
Entrez Gene: 8239 Human ; 22284 Mouse ; 363445 Rat
SwissProt: Q93008 Human ; P70398 Mouse ;
OMIM: 300919 Human
A synthesized peptide derived from human USP9x aa1-58/2750.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
USP9X is a Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-11'-, followed by 'Lys-63'-, 'Lys-48'- and 'Lys-6'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Deubiquitinates RNA demethylase enzyme ALKBH5, promoting its stability. Deubiquitinates mTORC2 complex component RICTOR at 'Lys-294' by removing 'Lys-63'-linked polyubiquitin chains, stabilizing RICTOR and enhancing its binding to MTOR, thus promoting mTORC2 complex assembly. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration. Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein BMAL1 via its deubiquitinating activity. Acts as a regulator of peroxisome import by mediating deubiquitination of PEX5: specifically deubiquitinates PEX5 monoubiquitinated at 'Cys-11' following its retrotranslocation into the cytosol, resetting PEX5 for a subsequent import cycle. Deubiquitinates PEG10. Inhibits the activation of the Hippo signaling pathway via deubiquitination of AMOTL2 at 'Lys-347' and 'Lys-408' which prohibits its interaction with and activation of LATS2. Loss of LATS2 activation and subsequent loss of YAP1 phosphorylation results in an increase in YAP1-driven transcription of target genes[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cytoplasm, cytosol; Cell projection, growth cone; Cytoplasm, cytoskeleton, cilium axoneme
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Expression
Tissue_specificity:Widely expressed in embryonic and adult tissues
Induction:By growth factors -
Isoforms & Post-Translational Modification
Q93008 has 2 isomers: Q93008-1: 290463 Da (predicted); Q93008-3: 292280 Da (predicted).
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Subunit
Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin (PubMed:16322459, PubMed:18254724, PubMed:19135894).
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SwissProt ID
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Synonyms
DFFRX; FAF; Fafl; Fam; hFAM; MRX99; Usp9x; X chromosome; X-linked
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Research Field
Cell Biology
Documentation
References
[1]. Al-Hakim AK, et al. Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains. Biochem J. 2008 Apr 15;411(2):249-60. [Content Brief]
[2]. Dupont S, et al. FAM/USP9x, a deubiquitinating enzyme essential for TGFbeta signaling, controls Smad4 monoubiquitination. Cell. 2009 Jan 9;136(1):123-35. [Content Brief]
[3]. Grou CP, et al. Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate. J Biol Chem. 2012 Apr 13;287(16):12815-27. [Content Brief]
[4]. Zhao Y, et al. Noncanonical regulation of alkylation damage resistance by the OTUD4 deubiquitinase. EMBO J. 2015 Jun 12;34(12):1687-703. [Content Brief]
[5]. Zhang Y, et al. Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1. Biochem J. 2018 Apr 30;475(8):1507-1522. [Content Brief]
[6]. Paudel P, et al. Crystal structure and activity-based labeling reveal the mechanisms for linkage-specific substrate recognition by deubiquitinase USP9X. Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7288-7297. [Content Brief]
[7]. Wang P, et al. Deubiquitinase USP9X stabilizes RNA m(6)A demethylase ALKBH5 and promotes acute myeloid leukemia cell survival. J Biol Chem. 2023 Aug;299(8):105055. [Content Brief]
[8]. Wrobel L, et al. mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR. Cell Rep. 2020 Dec 29;33(13):108564. [Content Brief]
[9]. Vong QP, et al. Chromosome alignment and segregation regulated by ubiquitination of survivin. Science. 2005 Dec 2;310(5753):1499-504. [Content Brief]
[10]. Homan CC, et al. Mutations in USP9X are associated with X-linked intellectual disability and disrupt neuronal cell migration and growth. Am J Hum Genet. 2014 Mar 6;94(3):470-8. [Content Brief]
[11]. Kim M, et al. Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition. EMBO Rep. 2016 Jan;17(1):64-78. [Content Brief]
[12]. Hwang D, et al. AMOTL2 mono-ubiquitination by WWP1 promotes contact inhibition by facilitating LATS activation. Life Sci Alliance. 2021 Oct;4(10):. [Content Brief]
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