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CDT2 Antibody (YA2474)

(Synonyms: CDW1; DCAF2; Dtl; L2DTL; RAMP)
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Based on 1 publication(s) in Google Scholar

CDT2 Antibody (YA2474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CDT2 Antibody (YA2474)
  • CDT2 Antibody (YA2474) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

CDT2 Antibody (YA2474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDT2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 79 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 79 kDa
Species Reactivity Database

Entrez Gene: 51514 Human

SwissProt: Q9NZJ0 Human

Immunogen

Recombinant protein of human CDT2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104827

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDT2 Antibody (YA2474)
    Western blot analysis of extracts from Hela(lane 2(20ug)，K562(lane 3(20ug) and HEK293T(lane 4(20ug) using CDT2 Antibody (HY-P82729) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    CDT2 is a Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1[1][2][3][4][5][6][7][8][9][10][11][12][13][14].

  • Subcellular Localization

    Nucleus; Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome

  • Expression


    Tissue_specificity:Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell

    Induction:Induced by TGF-beta, the up-regulation is immediate and transient

  • Isoforms & Post-Translational Modification

    Q9NZJ0 has 2 isomers: Q9NZJ0-1: 79468 Da (predicted); Q9NZJ0-2: 23682 Da (predicted).
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C). Autoubiquitinated through 'Lys-48'-polyubiquitin chains in a PCNA-independent reaction, allowing proteasomal turnover. Polyubiquitinated by SCF(FBXO11) when not phosphorylated, leading to its degradation. A tight regulation of the polyubiquitination by SCF(FBXO11) is involved in the control of different processes such as TGF-beta signaling, cell cycle progression and exit;Phosphorylated at Thr-464 by CDK1/Cyclin-B and CDK2/Cyclin-A but not by CDK2/Cyclin-E, MAPK1 or PLK1. Phosphorylation at Thr-464 inhibits the interaction with FBXO11 and decreases upon cell cycle exit induced by TGF-beta or serum starvation

  • Subunit

    Component of the DCX(DTL) E3 ubiquitin ligase complex (also called CRL4(CDT2)), at least composed of CUL4 (CUL4A or CUL4B), DDB1, DTL/CDT2 and RBX1 (PubMed:16861906, PubMed:16949367, PubMed:17041588, PubMed:17085480, PubMed:18794347, PubMed:18794348, PubMed:23478441). Interacts with CDKN1A (PubMed:23213251). Interacts with DDB1 (PubMed:16949367, PubMed:23478445). Interacts with FBXO11; SCF(FBXWO11) controls DTL stability but DCX(DTL) does not control FBXO11 stability (PubMed:23478441, PubMed:23478445). Interacts with CRY1 (PubMed:26431207)

  • SwissProt ID

    Q9NZJ0

  • Gene ID
    51514 [NCBI]
  • Synonyms

    CDW1; DCAF2; Dtl; L2DTL; RAMP

  • Research Field

    Cell Biology

CDT2 Antibody (YA2474) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，K562(lane 3(20ug) and HEK293T(lane 4(20ug) using CDT2 Antibody (HY-P82729) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDT2 Antibody (YA2474) Product Image
    1/16
  • Experimental Validation Results for CDT2 Antibody (YA2474)
  • CDT2 Antibody (YA2474) Product Image
  • Experimental Validation Results for CDT2 Antibody (YA2474)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，K562(lane 3(20ug) and HEK293T(lane 4(20ug) using CDT2 Antibody (HY-P82729) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDT2 Antibody (YA2474)
  • CDT2 Antibody (YA2474) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug)，K562(lane 3(20ug) and HEK293T(lane 4(20ug) using CDT2 Antibody (HY-P82729) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDT2 Antibody (YA2474) Product Image WB