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BMP6 Antibody (YA2048)

(Synonyms: BMP6; VGR; Bone morphogenetic protein 6; BMP-6; VG-1-related protein; VG-1-R; VGR-1)
Customer Review

Based on 1 Customer Validation

BMP6 Antibody (YA2048) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BMP6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • BMP6 Antibody (YA2048) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

BMP6 Antibody (YA2048) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BMP6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human BMP6 aa330-430/513.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104414

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Western blot analysis of extracts from Saos-2 (lane 2, 20ug), A549 (lane 3, 20ug), Mouse liver (lane 4, 20ug) and HEK293 (lane 5, 20ug) using BMP6 Antibody (HY-P82303). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    BMP6 is a Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes including cartilage and bone formation. Also plays an important role in the regulation of HAMP/hepcidin expression and iron metabolism by acting as a ligand for hemojuvelin/HJV. Also acts to promote expression of HAMP, potentially via the interaction with its receptor BMPR1A/ALK3. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. Can also signal through non-canonical pathway such as TAZ-Hippo signaling cascade to modulate VEGF signaling by regulating VEGFR2 expression[1][2][3][4][5][6].

  • Subcellular Localization

    Secreted

  • Subunit

    Interacts (when glycosylated) with type I receptor ACVR1; the interaction may induce HAMP expression (PubMed:18070108, PubMed:31800957).

  • SwissProt ID

    P22004

  • Gene ID
    654 [NCBI]
  • Synonyms

    BMP6; VGR; Bone morphogenetic protein 6; BMP-6; VG-1-related protein; VG-1-R; VGR-1

  • Research Field

    Cell Biology

BMP6 Antibody (YA2048) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane 2, 20ug), A549 (lane 3, 20ug), Mouse liver (lane 4, 20ug) and HEK293 (lane 5, 20ug) using BMP6 Antibody (HY-P82303). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    1/16
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • BMP6 Antibody (YA2048) Product Image
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane 2, 20ug), A549 (lane 3, 20ug), Mouse liver (lane 4, 20ug) and HEK293 (lane 5, 20ug) using BMP6 Antibody (HY-P82303). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • Experimental Validation Results for BMP6 Antibody (YA2048)
  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Saos-2 (lane 2, 20ug), A549 (lane 3, 20ug), Mouse liver (lane 4, 20ug) and HEK293 (lane 5, 20ug) using BMP6 Antibody (HY-P82303). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Spindle cell tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using BMP6 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82303, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • BMP6 Antibody (YA2048) Product Image WB
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P
  • BMP6 Antibody (YA2048) Product Image IHC-P