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alpha Synuclein Antibody (YA2073)(PBS only)

(Synonyms: SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP)

alpha Synuclein Antibody (YA2073) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Synuclein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

alpha Synuclein Antibody (YA2073) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Synuclein.

  • Host Rabbit
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Immunogen

Recombinant protein of human Alpha-synuclein(aa1-140).

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    alpha Synuclein is a Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Membrane; Nucleus; Synapse; Secreted; Cell projection, axon

  • Expression


    Tissue_specificity:It is highly expressed at presynaptic terminals in the central nervous system. It is mainly expressed in brain tissue.

  • Isoforms & Post-Translational Modification

    P37840 has 3 isomers: P37840-1: 14460 Da (predicted); P37840-2: 11372 Da (predicted); P37840-3: 13109 Da (predicted).
    Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress;Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers;Ubiquitinated. The predominant conjugate is the diubiquitinated form;Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure

  • Subunit

    Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800).

  • SwissProt ID

    P37840

  • Gene ID
    6622 [NCBI]
  • Synonyms

    SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

  • Research Field

    Neuroscience

alpha Synuclein Antibody (YA2073)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image
    1/16
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • Experimental Validation Results for alpha Synuclein Antibody (YA2073)(PBS only)
  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using alpha Synuclein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image IHC-P
  • alpha Synuclein Antibody (YA2073)(PBS only) Product Image IHC-P